Whole-cell lysates (WCL) were examined as we described36 (link). Nuclear and cytoplasmic proteins were extracted and analyzed as we recently reported5 (link). The following antibodies were used: c-MYC (clone 9E10; #sc-40, Santa Cruz Biotechnology), IDH2 (#ab55271, Abcam), OGT (#24083, Cell Signaling Technology), OGA (Cat #14711–1-AP, Proteintech), O-GlcNAc (clone RL2, #MA1–072, Thermo-Fisher Scientific), D2HGDH (#13895–1-AP, Proteintech), L2HGDH (#15707–1-AP, Proteintech), ALKBH5 (# ab69325, Abcam), FTO (c-3, #sc-271713, Santa Cruz Biotechnology), TET1 (#SAB2700730, Sigma-Aldrich), TET2 (#18950, Cell Signaling Technology), TET3 (#: ABS463, EMD Millipore Corporation), METTL3, METTL14, WTAP (# 69391, # 51104 and #56501, all from Cell Signaling Technology), β-actin (# A2228, Sigma-Aldrich), Lamin A (#A303–433A-M, Bethyl Laboratories), Tubulin (#62204 Invitrogen). PVDF membranes were stripped and re-probed with relevant antibodies for loading control, as reported37 (link).
O glcnac clone rl2
Manufactured by Thermo Fisher Scientific
The O-GlcNAc (clone RL2) is a monoclonal antibody that recognizes the O-linked N-acetylglucosamine (O-GlcNAc) modification on proteins. It is used for the detection and study of O-GlcNAcylated proteins in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
C myc clone 9e10, by Santa Cruz Biotechnology (2 mentions)
Alkbh5, by Abcam (2 mentions)
Mettl3, by Cell Signaling Technology (2 mentions)
D2hgdh, by Proteintech (2 mentions)
L2hgdh, by Proteintech (2 mentions)
Fto c 3, by Santa Cruz Biotechnology (2 mentions)
Mettl14, by Cell Signaling Technology (2 mentions)
Lamin a, by Fortis Life Sciences (2 mentions)
β actin, by Merck Group (2 mentions)
2 protocols using o glcnac clone rl2
1
Comprehensive Protein Analysis in Metabolic Disorders
2
Comprehensive Protein Analysis in Metabolic Disorders
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole-cell lysates (WCL) were examined as we described36 (link). Nuclear and cytoplasmic proteins were extracted and analyzed as we recently reported5 (link). The following antibodies were used: c-MYC (clone 9E10; #sc-40, Santa Cruz Biotechnology), IDH2 (#ab55271, Abcam), OGT (#24083, Cell Signaling Technology), OGA (Cat #14711–1-AP, Proteintech), O-GlcNAc (clone RL2, #MA1–072, Thermo-Fisher Scientific), D2HGDH (#13895–1-AP, Proteintech), L2HGDH (#15707–1-AP, Proteintech), ALKBH5 (# ab69325, Abcam), FTO (c-3, #sc-271713, Santa Cruz Biotechnology), TET1 (#SAB2700730, Sigma-Aldrich), TET2 (#18950, Cell Signaling Technology), TET3 (#: ABS463, EMD Millipore Corporation), METTL3, METTL14, WTAP (# 69391, # 51104 and #56501, all from Cell Signaling Technology), β-actin (# A2228, Sigma-Aldrich), Lamin A (#A303–433A-M, Bethyl Laboratories), Tubulin (#62204 Invitrogen). PVDF membranes were stripped and re-probed with relevant antibodies for loading control, as reported37 (link).
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