According to LPS (CSB-E09945h, CUSABIO, China), IL-1β (CSB-E08053h, CUSABIO, China), TNF-α (CSB-E04740h, CUSABIO, China), and IL-6 (CSB-E04638h, CUSABIO, China) ELISA instructions, we detected LPS level in feces and placental tissues and IL-6, IL-1β, and TNF-α levels in cells. 20 mg of feces and placental tissue was taken, respectively, and the blood stains were washed with 1 × PBS. Feces and placental tissue were cut into small pieces and put into tissue grinder (homogenate tube), 200 μL 1 × PBS was added to make homogenate, then put in -20°C overnight. After repeated freeze-thaw treatment two times to destroy the cell membrane, the tissue homogenate was centrifuged at 5000 g (2-8°C) for 5 min to take the supernatant, which should be detected. For cells, centrifugation was performed at 1000 g (2-8°C) for 15 min, and the supernatant should be immediately detected.
Csb e04638h
Manufactured by Cusabio
Sourced in China
The CSB-E04638h is a laboratory equipment product. It is designed to perform specific functions within a laboratory setting. A detailed description of its core function is not available at this time.
Automatically generated - may contain errors
3 protocols using csb e04638h
1
ELISA for Inflammatory Markers in Feces and Tissues
2
Cytokine Profiling in Healthy and CHF
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Blood samples were collected from 20 healthy subjects and 22 CHF patients. The level of IL-6 (CSB-E04638h, Cusabio biotech, China), IL-8 (CSB-E04641h, Cusabio biotech, China), IL-10 (CSB-E04593h, Cusabio biotech, China), and TNF-α (CSB-E04740h, Cusabio biotech, China) in the blood samples was determined by ELISA kit according to the manufacturer's instructions, and all samples analyses were repeated three times.
3
Measurement of Inflammatory Markers in Pregnant Rats
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The concentrations of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), and sFlt-1 in the cells, cell supernatant, or serum of pregnant rats were measured, according to the manufacturer's instructions. IL-6 (human, CSB-E04638h; rat, CSB-E04640r; Cusabio Biotech, China), TNF-α (human, CSB-E04740h; rat, CSB-E11987r; Cusabio Biotech, China), and sFlt-1 (human, ml038123; rat, ml059017; Mlbio, China) kits were used. Briefly, the samples were pretreated and incubated with an antibody working solution or enzyme at 37 °C for 1 h. Afterward, the samples were incubated with horseradish peroxidase-labeled working solution for 1 h at 37 °C or color developer for 15 min at 37 °C protected from light. Finally, the optical density (OD) value of each well was obtained at 450 nm via a multifunction enzyme analyzer (MB-530, Heales, China).
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