Sars spike glycoprotein

Manufactured by Abcam

Sourced in United States

The SARS spike glycoprotein is a recombinant protein derived from the spike (S) protein of the SARS-CoV-2 virus. The S protein is a key component of the virus that facilitates its entry into host cells. This purified protein can be used for various research applications, such as antibody detection, vaccine development, and COVID-19 diagnostic assays.

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Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (4 citations) Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (3 citations) Anti-SARS-CoV-2 spike glycoprotein (3 citations) Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (2 citations) SARS-Cov-2 Spike recombinant glycoprotein (ab49046) (2 citations)

3 protocols using sars spike glycoprotein

Immunocytochemistry of SARS-CoV-2 Markers

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Immunocytochemistry was carried out as previously described.²² (link) Briefly, the cells were fixed, permeabilized, blocked, and incubated with primary antibodies against SARS-CoV-2 nucleocapsid (1:100; GeneTex, Irvine, CA, USA), SARS spike glycoprotein (1:100; Abcam, Cambridge, UK), villin-1 (1:100; Cell Signaling Technology, Danvers, MA, USA), lysozyme (1:100; Thermo Fisher Scientific), mucin-2 (1:100; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and chr-A (1:100; Santa Cruz Biotechnology) at 4 °C. The cells were then incubated with Alexa 488-conjugated (1:200; Thermo Fisher Scientific) or Alexa 594-conjugated (1:200; Thermo Fisher Scientific) secondary antibodies for 1 h at room temperature. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole DAPI (Nacalai Tesque, Kyoto, Japan). The cells were mounted in SlowFade (Thermo Fisher Scientific) and examined under a confocal laser-scanning microscope (Nikon A1; Nikon, Tokyo, Japan).

Yamada S., Noda T., Okabe K., Yanagida S., Nishida M, & Kanda Y. (2022). SARS-CoV-2 induces barrier damage and inflammatory responses in the human iPSC-derived intestinal epithelium. Journal of Pharmacological Sciences, 149(3), 139-146.

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COVID-19 Serological Assay Development

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K₃Fe(CN)₆, K₄Fe(CN)₆, KCl, NaH₂PO₄, Na₂HPO₄, thionine, bovine serum albumin (BSA) and glutaraldehyde 25% solution in water were purchased from Sigma-Aldrich. COVID-19 spike protein and antibody were purchased from Sino Biological Inc. (Beijing, China). COVID-19 nucleocapsid protein, SARS spike glycoprotein and Human IgG protein were purchased from Abcam Australia Pty Ltd. All the chemicals were at least analytical grade and the water used for all experiments was purified by a Milli-Q system (resistivity > 18 MΩ·cm⁻¹). Phosphate buffer solution (PBS) used in this work was prepared using 0.1 M Na₂HPO₄·12H₂O, 0.1 M NaH₂PO₄·2H₂O, 0.1 M KCl, pH = 7.4.

Zhai Q., Wang X., Hu C., Zhu L., Zhang C, & Dai L. (2022). Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection. Sensors and Actuators Reports, 4, 100124.

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Comprehensive Immunocytochemical Staining Assay

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Cell staining was performed as previously described (Hirata et al., 2014) . Briefly, cells were fixed, permeabilized, blocked, and incubated with primary antibodies against SOX17 (1:500; R&D Systems), FOXA2 (1:1000; Cell Signaling Technology), CPM (1:400; Fujifilm Wako), TTF1/NKX2-1 (1:400; Abcam, Cambridge, UK), mature-SFTPB (1:500; Seven Hills Bioreagents, Cincinnati, OH, USA), pro-SFTPC (1:200; Abcam), SCGB3A2 (1:500; Abcam), and ACE2 (1:100; R&D systems), SARS-CoV-2 nucleocapsid (1:100; GeneTex, Irvine, CA, USA), or SARS spike glycoprotein (1:100; Abcam) in 5% FBS in PBS overnight at 4°C. After washing with PBS, cells were incubated with secondary antibodies, Alexa 488-conjugated anti-mouse IgG, Alexa 488-cojugated anti-goat IgG, and Alexa 594 -conjugated anti-rabbit IgG (1:200; Thermo Fisher Scientific) in 5% FBS for 1 hr at room temperature. Nuclei were counterstained with 4,6-Diamidino-2-phenylindole dihydrochloride (DAPI; Nacalai Tesque, Kyoto, Japan). Cells were mounted with VEC-TACHIELD mounting medium (Vector Laboratories, Burlingame, CA, USA). Fluorescence images were captured using a BIOREVO BZ-9000 fluorescence microscope (Keyence, Osaka, Japan) or a Nikon A1 confocal microscope (Nikon, Tokyo, Japan).

Tsuji K., Yamada S., Hirai K., Asakura H, & Kanda Y. (2021). Development of alveolar and airway cells from human iPS cells: toward SARS-CoV-2 research and drug toxicity testing. The Journal of toxicological sciences, 46(9).

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