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Lb broth

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LB broth is a widely used nutrient-rich medium for the growth of bacteria, particularly Escherichia coli (E. coli). It provides essential nutrients and supports the rapid proliferation of bacterial cultures in laboratory settings.

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Lab products found in correlation

Tetracycline, by Merck Group (1 mentions) Isopropyl β d thiogalactopyranoside, by Formedium (1 mentions) Glycerol, by Merck Group (1 mentions) Ampicillin, by Merck Group (1 mentions) Kanamycin, by Merck Group (1 mentions)

3 protocols using lb broth

1

Culturing B. thailandensis strains

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B. thailandensis strain E264 (GFP+) and B. thailandensis vgrG5ΔCTD (vgrG5 lacking the C-terminal domain required for fusion activity [6 (link)]), provided by Dr. Joseph Mougous (University of Washington, Seattle, WA), were cultured in LB broth (3002–031, MP Biomedicals, Santa Ana, CA) overnight and subcultured into fresh LB media for 3 h at 37°C to generate log-phase grown bacteria.
Place D.E., Briard B., Samir P., Karki R., Bhattacharya A., Guy C.S., Peters J.L., Frase S., Vogel P., Neale G., Yamamoto M, & Kanneganti T.D. (2020). Interferon inducible GBPs restrict Burkholderia thailandensis motility induced cell-cell fusion. PLoS Pathogens, 16(3), e1008364.
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2

Culturing Burkholderia thailandensis Strains

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B. thailandensis strain E264 (green fluorescent protein [GFP]+) and B. thailandensisvgrG5ΔCTD (vgrG5 lacking the C-terminal domain required for fusion activity) (65 (link)), provided by Joseph Mougous (University of Washington, Seattle, WA), were cultured in LB broth (3002-031, MP Biomedicals, Santa Ana, CA) overnight and subcultured into fresh LB medium for 3 h at 37°C to generate log-phase cultures.
Place D.E., Christgen S., Tuladhar S., Vogel P., Malireddi R.K, & Kanneganti T.D. (2021). Hierarchical Cell Death Program Disrupts the Intracellular Niche Required for Burkholderia thailandensis Pathogenesis. mBio, 12(3), e01059-21.
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3

Cultivation and Maintenance of Burkholderia Strains

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Burkholderia strains (Belgian Co-ordinated Collections of Micro-organisms) used in this study are listed in Table S1. Burkholderia and E. coli strains were routinely grown in LB broth (2.5%, MP Biomedicals) at 37 °C, with shaking at 200 rpm. Media were solidified with agar (1.5%, Invitrogen) and supplemented with filter-sterilized isopropyl-β-D-thiogalactopyranoside (IPTG, 25 μg/mL, ForMedium), ampicillin (100 μg/mL, Sigma-Aldrich), kanamycin (50 μg/mL, Sigma-Aldrich), or tetracycline (150 μg/mL, Sigma-Aldrich) when required. Bacterial strains were kept on plate at 4 °C, or stored at −80 °C in glycerol (25% v/v, Sigma-Aldrich).
Ghequire M.G, & De Mot R. (2015). Distinct colicin M-like bacteriocin-immunity pairs in Burkholderia. Scientific Reports, 5, 17368.
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