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Latrunculin b

Manufactured by Fujifilm
Sourced in Japan

Latrunculin B is a chemical compound used in laboratory research. It functions as an inhibitor of actin polymerization, a process essential for the formation and maintenance of the cytoskeleton in eukaryotic cells. Latrunculin B disrupts the cellular structure and can be used to study the role of the cytoskeleton in various biological processes.

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2 protocols using latrunculin b

1

Actin Depolymerization and Cell Wall Pits

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To transiently depolymerize actin microfilaments, 7-day-old wild-type seedlings were cultured on MS agar medium supplemented with 30 μM latrunculin B (Wako, 10 mM stock in DMSO) for 6 h. To examine the effect of actin disruption on the shape of secondary cell wall pits, 5-day-old wild-type seedlings were cultured on MS agar medium supplemented with 3 μM latrunculin B for 2 days.
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2

Signaling Pathways Antibody Detection

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Specific antibodies for pAkt(Thr308)(#2965), pAkt(Ser473)(#4051), Akt(#4691), pGSK3α/β(S21/9)(#9331), GSK3(#5676), p-ERK1/2(T202/Y204)(#4370), ERK1/2(#4695), and PTEN(#9559) were obtained from Cell Signaling Technology (MA). VO-OHpic and Wortmannin were obtained from Sigma Aldrich. Insulin, LatrunculinB, and LY294002 were obtained from Wako Pure Chemical Industries (Japan).
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