Trace 1300 gc

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

The Trace 1300 GC is a gas chromatograph designed for analytical laboratory applications. It features a compact design and is capable of performing highly sensitive, reproducible, and reliable gas chromatography analyses.

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Trace 1300 (145 citations) Trace GC (44 citations) TRACE 1300 GC system (4 citations) TRACE 1300 series GC (4 citations) TRACE-1300 series GC ultra-gas chromatograph (3 citations) Trace 1300-ISQ GC-MS system (3 citations) Thermo Trace 1300 GC (2 citations) Trace 1300 GC Ultra/Mass Spectrophotometer ISQ QD (2 citations) Trace GC 1300 gas chromatograph (2 citations) TRACE 1300-ISO GC–MS spectrometer (2 citations)

43 protocols using trace 1300 gc

Biogas Production Process Characterization

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Chemical oxygen demand (COD), total Kjeldahl nitrogen (TKN), ammonium nitrogen (NH4 + -), total solids (TS), volatile solids (VS) and pH were measured according to the standard methods (American Public Health Association (APHA), 2005). In both batch assays and CSTR operation, the methane concentration was measured twice per week using a gas chromatograph (GC-TRACE 1300, Thermo Scientific) (Khoshnevisan et al., 2018a) . The volumes of measured biogas were converted at standard temperature and pressure. Samples for VFA were collected from each reactor twice per week accompanying with pH measuring. pH was measured using a pH meter (Metrohm 744). VFA samples were measured using a gas chromatograph (GC-TRACE 1300, Thermo Scientific) (Khoshnevisan et al., 2018a) .
The trace elements were determined by inductively coupled plasma with optical emission spectrometry (ICP-OES).

Zha X., Tsapekos P., Alvarado-Morales M., Lu X, & Angelidaki I. (2020). Potassium inhibition during sludge and biopulp co-digestion; experimental and model-based approaches. Waste management (New York, N.Y.), 113.

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Dissolved Inorganic Carbon and Methane Analysis

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DIC water samples were analyzed using a DIC analyzer (Apollo SciTech, model AS-C5). The instrument was calibrated using repeated measurements of certified reference material from the Scripps Institution of Oceanography (CRM batch number 191). One LM vial for DIC measurement broke during the transportation and is therefore missing in the data. CH₄ water and sediment pore-water samples were quantified by headspace analysis using a gas chromatograph (GC Trace 1300, Thermo) equipped with an autosampler (TriPlus RSH, Thermo), a non-polar PLOT column (TracePLOT TG-BOND Q, Thermo), and a flame ionization detector. Certified standards of 1.86and 49.82 ppm CH₄ (Air Liquide Gas) were injected and used for calibration. The ppm concentrations were converted into molar concentrations, adjusting for sediment porosity, using the ideal gas law. Sediment porosity was determined by weighing wet and dry sediment (48 h at 70°C). This was followed by LOI analysis by measuring the weigh difference before and after igniting the dry sediment at 550°C for 5 h.

Broman E., Olsson M., Maciute A., Donald D., Humborg C., Norkko A., Jilbert T., Bonaglia S, & Nascimento F.J. (2024). Biotic interactions between benthic infauna and aerobic methanotrophs mediate methane fluxes from coastal sediments. The ISME Journal, 18(1), wrae013.

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GC-MS Analysis of P. nepalensis

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The GC-MS analysis of the roots and shoots of P. nepalensis was conducted using a Thermo Fisher Scientific Gas Chromatograph equipped with a Tri Plus RSH Autosampler, GC trace-1300, and MS-TSQ Duo. The Thermo Fisher Scientific TG-5MS column was utilized, which measured 40 m in length, 0.15 mm in film, and 0.15 m in internal diameter. The method involved setting the first oven temperature to 80 °C, with a temperature increase of 8 °C/min and a 1-min hold period, followed by increasing the temperature to 150 °C, with a rate of 10 °C/min and a 6-min hold period. The total run time was 32 min, with a 1 µL sample volume injected using helium at a flow rate of 0.7 mL/min as the carrier gas. The MS was operated within the electron ionization (EI) mode, scanning within a 40–450 amu range with a mass spectrometer source temperature and transfer line temperature set at 230 °C and 250 °C, respectively, and an electron multiplier voltage of 1 kV. Mass spectra were interpreted using the NIST/EPA/NIH Mass Spectral Library Version 2.2, 2014, and fragmentation patterns were compared with the instrument database data for all constituents detected.

Sharma S., Kumar V., Yaseen M., S. Abouzied A., Arshad A., Bhat M.A., Naglah A.M., Patel C.N., Sivakumar P.K., Sourirajan A., Shahzad A, & Dev K. (2023). Phytochemical Analysis, In Vitro Biological Activities, and Computer-Aided Analysis of Potentilla nepalensis Hook Compounds as Potential Melanoma Inhibitors Based on Molecular Docking, MD Simulations, and ADMET. Molecules, 28(13), 5108.

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Serum Fatty Acid Profiling by GC-MS/MS

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Gas chromatography-tandem mass spectrometry (GC-MS/MS) was used to analyze 37 fatty acids in serum, conducted in accordance with well-established protocols, which have been previously described in detail (Stupin et al., 2020 (link)). In brief, fatty acids were identified using the GC-MS/MS system by Thermo Fisher GC Trace 1300 coupled with a TSQ 9000 Triple Quadrupole (Thermo Fisher Scientific, Waltham, MA, USA), and the fatty acids profile was analyzed at the BIOCentre’s Bioanalytical Laboratory, BIOCentre－Incubation Center for Biosciences, Zagreb, Croatia.

Kolar L., Stupin M., Stupin A., Šušnjara P., Mihaljević Z., Matić A., Jukić I., Kolobarić N, & Drenjančević I. (2021). Does the Endothelium of Competitive Athletes Benefit from Consumption of n-3 Polyunsaturated Fatty Acid-Enriched Hen Eggs?. Preventive Nutrition and Food Science, 26(4), 388-399.

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Fatty Acid Analysis of Minced Meat

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The minced meat of the left MPS was freeze-dried in a lyophilization unit (Gamma 1-20, Christ^®, Osterode, Germany), pulverized in a mixer mill (MM 400, Retsch^®, Haan, Germany) and analyzed by gaschromatography (GC TRACE 1300, ThermoScientific^®, Dreieich, Germany; SP-2560 Column, Supelco, Bellefonte, USA; carrier gas: nitrogen) with a modified method according to Lepage and Roy [17 (link)]. In brief, a methanol-hexane-tridecanoic-acid mixture was utilized as standard. Subsequently, acetyl chloride was added and the sample was heated, followed by the addition of potassium chloride solution. The measurement was carried out after centrifugation with the superior hexane phase.

Zeiger K., Popp J., Becker A., Hankel J., Visscher C., Klein G, & Meemken D. (2017). Lauric acid as feed additive – An approach to reducing Campylobacter spp. in broiler meat. PLoS ONE, 12(4), e0175693.

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Comprehensive Anaerobic Digestion Analysis

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Alkalinity, pH, total solids (TS), volatile solids (VS) and chemical oxygen demand (COD) based on previous study (Tsapekos et al., 2017) . Volatile fatty acids (VFA) and methane content were monitored using gas chromatography GC-TRACE 1310 and GC-TRACE 1300 of Thermo Scientific, respectively, as previously described by Khoshnevisan et al. (2018) .
Methanogenic enzymatic activity (i.e. Coenzyme F420) was assayed adapting the classic spectrophotometric method (Miao et al., 2013) using a Synergy Mx multimode microplate reader (BioTek Instruments, Inc., USA). The autofluorescence was measured at an excitation wavelength of 425 nm and an emission wavelength of 472 nm. The TEs in inoculum and sewage sludge were analyzed by inductively coupled plasma with optical emission spectrometry (ICP-OES). For all analysis triplicate samples were measured.

Tsapekos P., Alvarado-Morales M., Tong J, & Angelidaki I. (2018). Nickel spiking to improve the methane yield of sewage sludge. Bioresource technology, 270.

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Gas Sampling and Analysis in H-Cell

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The headspaces of both septum-sealed
compartments of the H-cell shown in Scheme 2 were sampled every 15 min by using a lock-in
syringe with a push–pull valve (SGE Analytical Sciences). The
gas was then injected into a TRACE 1300 GC equipped with a thermal
conductivity detector (TCD) and an ISQ single quadrupole (mass spectrometry
detector, MSD) from Thermo Fisher Scientific (GC-TCD-MSD). The chromatographic
column was 5 Å molecular sieves, 80/100 mesh. Oxygen was detected
with the MSD, using helium as the carrier gas, and H₂ was
detected with the TCD, using argon as the carrier gas. The instrument
was calibrated for O₂ and H₂ by using 8.0% and
0.8% O₂ in N₂ or H₂ in N₂ standards (99%, Carbagas). Prior to injection, the injection line
was purged with N₂ and the exit of the line was connected
to a water trap in order to avoid suction of air during the injections.

Olaya A.J., Riva J.S., Baster D., Silva W.O., Pichard F, & Girault H.H. (2021). Visible-Light-Driven Water Oxidation on Self-Assembled Metal-Free Organic@Carbon Junctions at Neutral pH. JACS Au, 1(12), 2294-2302.

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GC-MS Analysis of Derivatized Samples

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MOX + TMS derivatized samples were analyzed by GC-MS. 1 μL of derivatized sample was injected by autosampler into a Trace 1300 GC (ThermoFisher Scientific) fitted with a TraceGold TG-5SilMS column (ThermoFisher Scientific) operating under the following conditions: split ratio = 20:1, split flow = 24 μL/min, purge flow = 5 mL/min, carrier mode = Constant Flow, and carrier flow rate = 1.2 ml/min. The GC oven temperature gradient was as follows: 80 °C for 3 min, increasing at a rate of 20 °C/min to 280 °C, and holding at a temperature at 280 °C for 8 min. Between sample runs, the autosampler injection syringe was washed 3 times with methanol and 3 times with pyridine. Ion detection was performed by an ISQ 7000 mass spectrometer (ThermoFisher Scientific) operated from 3.90 to 21.00 min in EI mode (-70eV) using select ion monitoring (SIM). The mass spectrometer was tuned and calibrated daily. Samples were analyzed in a randomized order, and the pooled sample detailed above was analyzed at the beginning of, at a set interval (about every eight injections) during, and the end of the analytical run.

Li Q., Zhang Q., Kim Y.R., Gaddam R.R., Jacobs J.S., Bachschmid M.M., Younis T., Zhu Z., Zingman L., London B., Rauckhorst A.J., Taylor E.B., Norris A.W., Vikram A, & Irani K. (2023). Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome. Nature Communications, 14, 5595.

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Compounds Identification in Ethyl Acetate Extract

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To identify the compounds in ethyl acetate extract GC-MS analysis was performed using THERMO Trace 1300GC coupled with THERMO TSQ8000 Triple Quadrupole MS (column BP-5MS). The instrument was firstly set to an initial temperature of 50 °C followed by raise in temperature up to 200 °C, at a rate of 10 °C/min with a holding time of 5 min. Further, the temperature was increased to 260 °C at a rate of 10 °C/min with a holding time of 5 min. The injection port temperature was maintained at 260 °C and the following parameters, flow rate of helium gas at 1.0 ml/minute, ionization voltage of 70 ev, 15:1 split ratio and mass spectral scans range of 35–650 (m/z) were used. Compounds were identified by using computer searches on a NIST Ver.2.1 MS data repository and by comparison of the spectra acquired using GC–MS.

Sharma A., Chauhan P., Sharma K., Kalotra V., Kaur A., Chadha P., Kaur S, & Kaur A. (2022). An endophytic Schizophyllum commune possessing antioxidant activity exhibits genoprotective and organprotective effects in fresh water fish Channa punctatus exposed to bisphenol A. BMC Microbiology, 22, 291.

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Metabolite Derivatization and GC-MS Analysis

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The dried samples, QC sample, and processing blank sample, were derivatized using methoxyamine hydrochloride (MOX) and N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) (Sigma). Briefly, dried extracts were reconstituted in 30 μL of 11.4 mg/ml MOX in anhydrous pyridine (VWR), vortexed for 10 minutes, and heated at 60°C for 1 hour. Next, 20 μL TMS was added to each reconstituted extract, vortexed for 1 minute, and heated at 60°C for 30 min. The derivatized samples, QC samples and processing blank samples were immediately analyzed using GC/MS.
GC chromatographic separation was conducted on a Thermo Trace 1300 GC with a TraceGold TG-5SilMS column (0.25 μM film thickness; 0.25mm ID; 30 m length). 1 μL of derivatized sample, QC, or blank was injected. The GC was operated in split mode with the following settings: 20:1 split ratio; split flow: 24 μL/min, purge flow: 5 mL/min, Carrier mode: Constant Flow, Carrier flow rate: 1.2 mL/min). The GC inlet temperature was 250°C. The GC oven temperature gradient was as follows: 80°C for 3 min, ramped at 20°C/min to a maximum temperature of 280°C, which was held for 8 min. The injection syringe was washed 3 times with pyridine between each sample. Metabolites were detected using a Thermo ISQ single quadrupole mass spectrometer. The data was acquired from 3.90 to 21.00 min in EI mode (70eV) by single ion monitoring (SIM).

Buchanan J.L., Rauckhorst A.J, & Taylor E.B. (2023). 3-hydroxykynurenine is a ROS-inducing cytotoxic tryptophan metabolite that disrupts the TCA cycle. bioRxiv.

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