Cxcr4 antibody

Manufactured by Boster Bio

Sourced in China

The CXCR4 antibody is a laboratory reagent used for the detection and analysis of the CXCR4 protein. CXCR4 is a chemokine receptor that plays a crucial role in various cellular processes. The antibody can be used in techniques such as flow cytometry, immunohistochemistry, and Western blotting to study the expression and localization of CXCR4 in biological samples.

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Lab products found in correlation

Bovine serum albumin (bsa), by Boster Bio (1 mentions) Radioimmunoprecipitation assay (ripa), by Boster Bio (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Keratinocyte serum free medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) P53 antibody, by Santa Cruz Biotechnology (1 mentions) N n hexamethylenebisacrylamide hmba, by Polysciences (1 mentions) Cyclam, by Thermo Fisher Scientific (1 mentions)

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CXCR4 (2 citations)

2 protocols using cxcr4 antibody

Quantification of MMP2 and CXCR4 Proteins

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Appropriate amount of RIPA (BOSTER, China) was added to the transfected cells and spinal cord tissues for 3–5 min to completely lyse the cells and tissues, and the supernatant was collected after centrifugation for the determination of total protein using the BCA protein concentration kit (BOSTER, China). The same amount of protein was separated by SDS-PAGE, and the resolved proteins were transferred to a PVDF membrane. The membrane was then blocked with 5% bovine serum albumin (BOSTER, China) for 1.5 hours, and sequentially incubated with primary antibodies overnight at 4°C (MMP2 antibody dilution ratio 1:10000, CXCR4 antibody dilution ratio 1:1000, BOSTER, China), and second antibody (goat anti-rabbit IgG labelled with HRP, dilution 1:5000, absin, China) at room temperature. Western blot analyses were repeated for three times.

Zhang Y., Cao L., Du R., Tian F., Li X., Yuan Y, & Wang C. (2022). MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells. PLoS ONE, 17(9), e0272499.

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Optimizing HMBA-Mediated Gene Delivery

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N,N′-hexamethylenebisacrylamide (HMBA) was obtained from Polysciences, Inc. Cyclam (1,4,8,11-tetraazacyclotetradecane) was ordered from Alfa Aesar. Dulbecco’s modified Eagle medium (DMEM), Dulbecco’s phosphate buffered saline (PBS), and FBS were from Thermo Scientific. Cell culture plates and flasks were ordered from BD Biosciences. Human SDF-1α was purchased from Shenandoah Biotechnology, Inc. Keratinocyte Serum Free Medium (K-SFM) and its supplements were ordered from Gibco. RPMI-1640 medium was purchased from Gibco. Pre-designed p53 siRNA was ordered from Sigma Aldrich. Cy5.5 labeled siRNA (siRNA-Cy5.5) were from Dharmacon. Real-time PCR (RT-PCR) primers were ordered from Invitrogen. CXCR4 antibody was ordered from Boster Biological Technology. p53 antibody was ordered from Santa Cruz Biotechnology, Inc. All other reagents were from Fisher Scientific and used as received unless otherwise noted.

Tang W., Chen Y., Jang H.S., Hang Y., Jogdeo C.M., Li J., Ding L., Zhang C., Yu A., Yu F., Foster K.W., Padanilam B.J, & Oupický D. (2021). Preferential siRNA delivery to injured kidneys for combination treatment of acute kidney injury. Journal of controlled release : official journal of the Controlled Release Society, 341, 300-313.

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