Primer script reverse transcriptase reagent kit with gdna eraser

Manufactured by Takara Bio

The Primer Script Reverse Transcriptase Reagent Kit with gDNA Eraser is a laboratory product designed for the synthesis of first-strand cDNA from RNA templates. The kit includes a reverse transcriptase enzyme, buffer solutions, and a genomic DNA (gDNA) eraser component to remove any contaminating gDNA from the RNA sample prior to cDNA synthesis.

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Lab products found in correlation

Cfx96 thermal cycler, by Bio-Rad (2 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Tb green premix ex taq, by Takara Bio (1 mentions)

Spelling variants of this product

GDNA Eraser (796 citations) Reverse transcriptase (314 citations) Reverse transcriptase kit (303 citations) GDNA Eraser kit (104 citations) Primer Script Kit (6 citations) Reverse primer (6 citations) Primer Script Reverse (2 citations)

2 protocols using primer script reverse transcriptase reagent kit with gdna eraser

Isolation and Real-Time PCR Analysis

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Total RNA was isolated using Trizol (Invitrogen, 15596018) according to the manufacturer’s instructions. RNA was reversely transcribed using Primer Script Reverse Transcriptase Reagent Kit with gDNA Eraser (Takara, RR036A). Real-time PCR was performed using the SYBR Premix kit (Genstar, A301) and analyzed using the Bio-Rad CFX96 thermal cycler. The primer sequences used for the investigated mouse genes are listed in online supplemental table 1.

Wang Z., Xu F., Hu J., Zhang H., Cui L., Lu W., He W., Wang X., Li M., Zhang H., Xiong W., Xie C., Liu Y., Zhou P., Liu J., Huang P., Qin X.F, & Xia X. (2021). Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity. Journal for Immunotherapy of Cancer, 9(5), e002155.

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Profiling Immune-Related lncRNA in NSCLC

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We collected sixteen paired NSCLC and adjacent normal tissue samples from Jiangxi Cancer Hospital after gaining ethical approval from the Human Research Ethics Committee in Jiangxi Cancer Hospital. Total RNA was isolated using RNAiso Plus (Takara) according to the manufacturer’s instructions. The RNA was reverse-transcribed using the Primer Script Reverse Transcriptase Reagent Kit with gDNA Eraser (Takara, RR047A). Real-time PCR was performed using the TB Green™ Premix Ex Taq™ (Takara, RR420A) and analyzed using the Bio-Rad CFX96 thermal cycler. The primer sequences used for the investigated genes are listed in online Supplementary Table 3. GADPH was used to standardize the gene expression. In order to compare the expression levels of lncRNA in different samples, the 2^-ΔΔCt method was adopted to calculate the expression levels of the immune-related lncRNA from the risk model.

Li Q., Yao L., Lin Z., Li F., Xie D., Li C., Zhan W., Lin W., Huang L., Wu S, & Zhou H. (2021). Identification of Prognostic Model Based on Immune-Related LncRNAs in Stage I-III Non-Small Cell Lung Cancer. Frontiers in Oncology, 11, 706616.

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