Arp3 mcherry
ARP3-mCherry is a fluorescent protein construct that contains the ARP3 (Actin-Related Protein 3) gene fused to the mCherry fluorescent protein. ARP3 is a subunit of the Arp2/3 complex, which plays a crucial role in the regulation of actin cytoskeleton dynamics. The mCherry fluorescent tag allows for the visualization and tracking of the ARP3 protein in live cells.
Lab products found in correlation
2 protocols using arp3 mcherry
Fluorescently Tagged Podosome Proteins
Fluorescence Imaging of Podosome Proteins
CMV-GFP-NMHCII-A was a gift from Robert Adelstein (Addgene #11347). ARP3-mCherry (Addgene #27682) and mCherry-cortactin (Addgene #27676) were gifts from Christien Merrifield that were subcloned into EGFP-C1 and mEmerald-C1, respectively. Chicken paxillin was a gift from Chris Turner, which was subcloned into mScarlet-i-C1. Chicken vinculin was a gift from Kenneth Yamada (Addgene #50513) and subcloned into pUB-mEmerald-C1.
Immunohistochemical staining to determine localization of select podosome-related proteins in relation to the actin teeth did not produce good results, which may be due to the adhesive and porous nature of the IgG-functionalized DAAMPs. As an alternative, we transfected the RAW macrophages with fluorescently tagged proteins. All transfections were accomplished by electroporation (Neon) using the manufacturer's instructions.
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