Nebnext ultra 2 non directional rna library prep kit for

RNA quality, three per condition, was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA); with RIN > 7. Sample processing was carried out at the Division Core Facility for Molecular Biology at the Centre of Medical Research at the Medical University of Graz. Briefly, ribosomal-depletion was performed with NEBnext rRNA Depletion Kit V2 (New England Biolabs; Ipswich, MA, USA; Cat.No. E7405) and the library was prepared with NEBnext Ultra II (non directional) RNA Library Prep Kit for Illumina (New England Biolabs; Ipswich, MA, USA; Cat.No. E7770). Index were used from NEBNext Multiplex Oligos, Set 1 (New England Biolabs; Ipswich, MA, USA; Cat.No. E7335S). The RNA-Seq was performed at Vienna BioCenter Core Facilities GmbH, samples were indexed and sequenced on an Illumina NovaSeq SP SR100 XP (single lane). The generated RNA-Seq raw data are accessible through NCBI Short Read Archive under the accession number PRJNA904961.