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Anti cd123 percp cy5.5 clone 7g3

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The Anti-CD123 PerCP-Cy5.5 (clone 7G3) is a monoclonal antibody conjugated with PerCP-Cy5.5 fluorochrome. It is designed to detect the CD123 antigen, which is expressed on the surface of various cell types.

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Lab products found in correlation

Alexa fluor 488 antibody, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm kit, by BD (2 mentions) Anti cd20 pe cf594 clone 2h7, by BD (2 mentions) Anti cd3 bv421 clone sp34 2, by BD (2 mentions) Anti cd16 bv605 clone sg8, by BD (2 mentions) Anti cd4 bv711 clone l200, by BD (2 mentions) Anti cd14 bv786 clone m5e2, by BD (2 mentions) Anti cd8 pe cy7 clone sk1, by BD (2 mentions) Anti cd11c alexa700 clone 3.9, by Thermo Fisher Scientific (2 mentions) Anti hla dr apc cy7 clone l243, by BD (2 mentions) Anti p100 clone epr18756, by Abcam (2 mentions) 4 laser fortessa flow cytometer, by BD (2 mentions) Facsaria 2, by BD (1 mentions) Anti hla dr alexa700 clone l243, by BioLegend (1 mentions) Anti cd14 pedy594 clone mem 15, by Exbio (1 mentions)

Close spelling variants of this product

PerCP-Cy5.5 (120 citations) CD123-PerCP-Cy5.5 (12 citations) CD123 (7G3; (4 citations) Anti-CD123 (7G3, (3 citations) Anti-CD123 (Clone 7G3) (2 citations)

3 protocols using anti cd123 percp cy5.5 clone 7g3

1

Multiparameter Flow Cytometric Immune Profiling

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Whole blood collected in Cyto-Chex® blood collection tubes (Myriad RBM) was added to ACK lysis buffer to remove red blood cells, then washed with PBS and resuspended in a staining cocktail including anti-CD3 BV421 (clone SP34-2; BD Biosciences), anti-CD16 BV605 (clone SG8; BD Biosciences), anti-CD4 BV711 (clone L200; BD Biosciences), anti-CD14 BV786 (clone M5E2; BD Biosciences), anti-CD123 PerCP-Cy5.5 (clone 7G3; BD Biosciences), anti-CD20 PE-CF594 (clone 2H7; BD Biosciences), anti-CD8 PE-Cy7 (clone SK1; BD Biosciences), anti-CD11c Alexa700 (clone 3.9; Ebioscience), and anti-HLA-DR APC-Cy7 (clone L243; BD Biosciences). Following surface staining, cells were washed twice with phosphate-buffered saline (PBS), then permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, kit used as directed), and stained intracellularly with anti-p100 (clone EPR18756; Abcam). Cells were washed twice and stained with a secondary chicken anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Samples were acquired using a 4-laser Fortessa™ flow cytometer (BD Biosciences) and analyzed with FlowJo™ software (version 9.7.6, Treestar).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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2

Profiling PD-L1 expression in PBMCs

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PBMCs were cultured in complete media with or without rhIL-27 (100 ng/ml) overnight at 37 °C. Then, the cells were washed and stained with FITC-conjugated antibodies against lineage-specific markers (CD3 clone MEM-57, CD19 clone LT19, CD20 clone LT20, CD16 clone LNK16, and CD56 clone MEM-188), anti-CD14-PEDy594 (clone MEM-15), anti-CD11c-PB (clone BU15) (Exbio), anti-CD123-PerCP-Cy5.5 (clone 7G3) (BD Biosciences), anti-HLA-DR-Alexa700 (clone L243), anti-PD-L1-PC7 (clone 29E.2A3), and anti-CD86-Alexa647 (clone IT2.2) (BioLegend, San Diego, USA). The samples were acquired on a FACSAria II (BD Biosciences), and data analysis was performed using FlowJo software (TreeStar). Next, the expression of PD-L1 in PBMCs was analyzed by RT-PCR after 100 ng/ml IL-27 stimulation for 5 hours.
Parackova Z., Vrabcova P., Zentsova I., Kayserova J., Richtrova I., Sojka L., Stechova K., Sumnik Z, & Sediva A. (2020). Enhanced STAT3 phosphorylation and PD-L1 expression in myeloid dendritic cells indicate impaired IL-27Ralpha signaling in type 1 diabetes. Scientific Reports, 10, 493.
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3

Multiparameter Flow Cytometric Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole blood collected in Cyto-Chex® blood collection tubes (Myriad RBM) was added to ACK lysis buffer to remove red blood cells, then washed with PBS and resuspended in a staining cocktail including anti-CD3 BV421 (clone SP34-2; BD Biosciences), anti-CD16 BV605 (clone SG8; BD Biosciences), anti-CD4 BV711 (clone L200; BD Biosciences), anti-CD14 BV786 (clone M5E2; BD Biosciences), anti-CD123 PerCP-Cy5.5 (clone 7G3; BD Biosciences), anti-CD20 PE-CF594 (clone 2H7; BD Biosciences), anti-CD8 PE-Cy7 (clone SK1; BD Biosciences), anti-CD11c Alexa700 (clone 3.9; Ebioscience), and anti-HLA-DR APC-Cy7 (clone L243; BD Biosciences). Following surface staining, cells were washed twice with phosphate-buffered saline (PBS), then permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, kit used as directed), and stained intracellularly with anti-p100 (clone EPR18756; Abcam). Cells were washed twice and stained with a secondary chicken anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Samples were acquired using a 4-laser Fortessa™ flow cytometer (BD Biosciences) and analyzed with FlowJo™ software (version 9.7.6, Treestar).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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