Human pancreatic cancer tissue arrays were stained by standard IHC with NAE1 and NEDD8 antibodies by Shanghai Biochip, Shanghai, China. Briefly, the tissue array sections (5 microns) were dehydrated and subjected to peroxidase blocking. Primary antibodies were added and incubated at room temperature for 30 min on the DAKO AutoStainer using the Dako-Cytomation EnVision + System-HRP (DAB) detection kit. The slides were counterstained with hematoxylin. The staining images were acquired under microscopy, and staining intensity was scored from weak (+) to very strong (++++).
Envision system hrp dab detection kit
Manufactured by Agilent Technologies
The EnVision + System-HRP (DAB) detection kit is a laboratory equipment product from Agilent Technologies. It is designed for the detection of target proteins in immunohistochemistry (IHC) and other immunoassay applications. The kit utilizes the highly sensitive horseradish peroxidase (HRP) enzyme system and the 3,3'-diaminobenzidine (DAB) chromogen to enable visualization of the target proteins.
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Lab products found in correlation
2 protocols using envision system hrp dab detection kit
1
Immunohistochemical Analysis of NAE1 and NEDD8 in Human Pancreatic Cancer
2
Immunohistochemical Profiling of RPS27L in Breast Tissue
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Human breast tissue microarrays were provided and stained with anti-RPS27L antibody13 (link) by the University of Michigan Comprehensive Cancer Tissue Core. Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the arrays were incubated with RPS27L antibody at room temperature for 30 min on the DAKO AutoStainer using the DakoCytomation EnVision + System-HRP (DAB) detection kit, followed by counterstaining with hematoxylin. The stained slides were observed under a microscope (Olympus 1 × 71) and images were acquired using software DP controller (ver. 3.1.1.267, Olympus). Stained tissues were classified into four groups according to the staining intensity of each tissue.
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