Anti total p53

Manufactured by Cell Signaling Technology

Anti-total p53 is a laboratory reagent that detects the total p53 protein. It is intended for use in research applications involving the analysis of p53 expression and localization.

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2 protocols using anti total p53

Comprehensive Immunoblotting Approach

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Western immunoblots were run as described previously 21 (link). Primary antibodies and their sources were as follows. Anti-total p53, anti-cyclin B1, anti-Bax, anti-Bcl-2, anti-CDK1, anti-phospho-CDK1, anti-activated caspase 8, anti-activated caspase 9, and anti-activated caspase 3 were from Cell Signaling Technology. Anti-ZIKV Env monoclonal antibody was purchased from Zoongen Co., Ltd (Beijing, China). Anti-p21^Cip1/Waf1 and anti-6×His tag was from Abcam. Anti-β-actin and the secondary antibodies horseradish peroxidase-conjugated anti-rabbit IgG and anti-rabbit IgG were from Sigma-Aldrich. Anti-goat IgG was from Santa Cruz Biotechnology.

Liu J., Li Q., Li X., Qiu Z., Li A., Liang W., Chen H., Cai X., Chen X., Duan X., Li J., Wu W., Xu M., Mao Y., Chen H., Li J., Gu W, & Li H. (2018). Zika Virus Envelope Protein induces G2/M Cell Cycle Arrest and Apoptosis via an Intrinsic Cell Death Signaling Pathway in Neuroendocrine PC12 Cells. International Journal of Biological Sciences, 14(9), 1099-1108.

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Western Blot Antibody Detection Protocol

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Western immunoblots were run as described previously [15] (link). Primary antibodies and their sources were as follows. Anti-total p38 MAPK, anti-total p53, anti-total p44/42 MAPK, anti-phosphorylated p44/42 (pp44/42) MAPK, and anti-phosphorylated p53 (pp53) were from Cell Signaling Technology. Anti-phosphorylated p38 (pp38) MAPK was from Millipore. Anti-β-actin and the secondary antibodies horseradish peroxidase-conjugated anti-rabbit IgG and anti-rabbit IgG were from Sigma-Aldrich. Anti-goat IgG was from Santa Cruz Biotechnology.

Pei N., Jie F., Luo J., Wan R., Zhang Y., Chen X., Liang Z., Du H., Li A., Chen B., Zhang Y., Sumners C., Li J., Gu W, & Li H. (2014). Gene Expression Profiling Associated with Angiotensin II Type 2 Receptor-Induced Apoptosis in Human Prostate Cancer Cells. PLoS ONE, 9(3), e92253.

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