Maxwell rsc automated instrument

Liquid biopsy was conducted to collect patient samples. Peripheral blood from the patients was obtained through venipuncture immediately prior to surgery, yielding a total of 8 mL of blood using EDTA tubes. Two successive centrifugation steps were performed to separate plasma from serum. The first centrifugation was carried out at 1,200 × g for 10 min, followed by a second centrifugation at 16,000 × g for 15 min at 4°C. Subsequently, 1 mL of the plasma was processed using the Maxwell^® RSC automated instrument from Promega Corporation (USA,) following the manufacturer's protocols in order to isolate cfDNA from the plasma. The cfDNA samples were then quantified using the Quantus™ Fluorometer (Promega Corporation, USA).

Genomic DNA was extracted from peripheral blood samples using Maxwell RSC automated instrument (Promega) according to the manufacturer’s protocol. Purified DNA was quantitated making use of Quant-iT PicoGreen dsDNA reagent (Invitrogen).

Maxwell RSC automated instrument (promega) was used to carry out genomic DNA extraction from peripheral blood samples, following the protocol of the manufacturer. Quantification of purified DNA was performed with Quant-iT PicoGreen dsDNA reagent (Invitrogen).