Hace2

Manufactured by R&D Systems

Sourced in United States

HACE2 (Histone Acetyltransferase Complex Subunit) is a lab equipment product that serves as a tool for biological research. It is a key component in the study of epigenetic regulation, particularly the role of histone acetylation in gene expression and chromatin structure.

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Lab products found in correlation

Anti hace2, by R&D Systems (1 mentions) Tetramethylbenzidine tmb substrate, by BD (1 mentions) High binding microplate, by Greiner (1 mentions) Tetramethylbenzidine, by BD (1 mentions) Spectramax id3x microplate reader, by Molecular Devices (1 mentions)

Spelling variants of this product

Anti-hACE2 (5 citations) Anti-hACE2 antibodies (4 citations) HACE2-specific goat antibody (3 citations)

2 protocols using hace2

ACE2-RBD Binding Quantification by ELISA

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ACE2 binding to the produced RBD variant antigens was analyzed by ELISA. Briefly, 0.1 ug of RBD per well was immobilized on a high binding Microplate (Greiner Bio-One GmbH, Austria.) and incubated ON at 4 °C. Next, plates were blocked with 5% non-fat milk in PBS 0.05% Tween20 for 2 h at 37 °C and then washed with PBS and 0.05% Tween20. Plates were then incubated with hACE2 (R&D, Minneapolis MN, USA) for 2 h at 37 °C and another washing step was performed. Finally, plates were incubated with anti-hACE2 (R&D Systems, Cat#: AF933. Lot: HOK0620051) diluted to a concentration of 0.5 µg/ml in 1% non-fat milk PBS 0.05% Tween 20 for 2 h at 37 °C. Detection was performed with a secondary Ab conjugated to HRP (Agilent DAKO, Santa Clara, CA, USA, Cat# P0449, Lot:41308941) at a dilution: 1/1000 in 1% non-fat milk PBS 0.05% Tween 20 for 1 h at 37 °C and visualized with tetramethylbenzidine (TMB) substrate (BD biosciences, Franklin Lakes, New Jersey, USA).

Coria L.M., Rodriguez J.M., Demaria A., Bruno L.A., Medrano M.R., Castro C.P., Castro E.F., Del Priore S.A., Hernando Insua A.C., Kaufmann I.G., Saposnik L.M., Stone W.B., Prado L., Notaro U.S., Amweg A.N., Diaz P.U., Avaro M., Ortega H., Ceballos A., Krum V., Zurvarra F.M., Sidabra J.E., Drehe I., Baqué J.A., Li Causi M., De Nichilo A.V., Payes C.J., Southard T., Vega J.C., Auguste A.J., Álvarez D.E., Flo J.M., Pasquevich K.A, & Cassataro J. (2024). A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection. Nature Communications, 15, 997.

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Surrogate Virus Neutralization Assay

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The surrogate virus neutralization test was performed by modification of a previously published protocol (Tan et al., 2020 (link)). Briefly, high binding flat bottom 96-well plates were coated with 2 μg/ml recombinant human ACE2 (hACE2, MilliporeSigma) in PBS, incubated overnight at 4°C, washed three times with PBST and blocked with PBS + BSA 1% for 1 hour at room temperature. In the meantime, each serum sample (final dilution 1:160) was pre-incubated with 3 ng of RBD-Fc (R&D Systems) in PBS + BSA 1% for 1 hour at room temperature and then transferred to the hACE2-coated plate. As positive control, RBD-Fc was also added to hACE2-coated wells without pre-incubation with serum samples. After 1 hour at room temperature, plates were washed three times with PBST and incubated with an HRP-conjugated anti-human IgG Fc antibody (Southern Biotech) for 1 hour at room temperature. At the end of the incubation, plates were washed five times with PBST and developed with tetramethylbenzidine (BD Biosciences) for 5 min, then stopped with 2 N H₂SO₄. The optical density was read at 450 nm with SpectraMax iD3x microplate reader (Molecular Devices). Percentage inhibition of RBD binding to hACE2 was calculated with the following formula: Inhibition (%) = [1 - (Sample OD value - Background OD value) / (Control OD value - Background OD value)] x 100.

Borriello F., Poli V., Shrock E., Spreafico R., Liu X., Pishesha N., Carpenet C., Chou J., Di Gioia M., McGrath M.E., Dillen C.A., Barrett N.A., Lacanfora L., Franco M.E., Marongiu L., Iwakura Y., Pucci F., Kruppa M.D., Ma Z., Lowman D.W., Ensley H.E., Nanishi E., Saito Y., O’Meara T.R., Seo H.S., Dhe-Paganon S., Dowling D.J., Frieman M., Elledge S.J., Levy O., Irvine D.J., Ploegh H.L., Wiliams D.L, & Zanoni I. (2022). An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity. Cell, 185(4), 614-629.e21.

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