Four microliter of the samples at a concentration of 1 µg/µl was applied onto Quantifoil R2/2 300-mesh holey carbon grid, coated with thin home-made continuous carbon film and glow-discharged (3 mA for 20 s). The sample was incubated on the grid for 30 s and then blotted with filter paper for 2.5 s in a temperature and humidity controlled Vitrobot Mark IV (T = 4 °C, humidity 100%, blot force 5) followed by vitrification in liquid ethane.
R2 2 300 mesh holey carbon grid
Manufactured by Quantifoil
The R2/2 300-mesh holey carbon grid is a specialized laboratory equipment designed for electron microscopy applications. It features a carbon film with a regular array of precisely spaced holes, providing a stable and uniform substrate for samples under examination. The grid's 300-mesh configuration ensures a consistent and controlled sample environment for high-resolution imaging and analysis.
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Lab products found in correlation
5 protocols using r2 2 300 mesh holey carbon grid
1
Cryo-EM Sample Preparation Protocol
2
Vitrification of Protein Samples
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For the single-particle analyses, 4 µl of the samples at a protein concentration of 1.5 µg/µl was applied onto Quantifoil R2/2 300-mesh holey carbon grid, coated with thin home-made continuous carbon film and glow-discharged (2.5 mA for 20 s). The sample was incubated on the grid for 30 s and then blotted with filter paper for 2 s in a temperature and humidity-controlled Vitrobot Mark IV (T = 4 °C, humidity 100%, blot force 5) followed by vitrification in liquid ethane.
3
Cryo-EM Sample Preparation Protocol
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Here 4 µL of sample at a concentration of 2 µg/µL was applied onto Quantifoil R2/2 300-mesh holey carbon grid, which had been coated with a thin home-made continuous carbon film and glow-discharged. The sample was incubated on the grid for 30 s and then blotted with filter paper for 2 s in a temperature- and humidity-controlled Vitrobot Mark IV (4 °C; 100% humidity; blot force 5), followed by vitrification in liquid ethane precooled by liquid nitrogen.
4
Grid Preparation for Cryo-EM Sample
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Grid preparation: 4 μL of the sample at a concentration of 90 nM was applied onto the Quantifoil R2/2 300-mesh holey carbon grid, which had been coated with thin carbon film (about 2nm) and glow-discharged. The sample was incubated on the grid for 30 s and then blotted with filter paper for 1.5 s in a temperature and humidity controlled Vitrobot Mark IV (T = 4°C, humidity 100%, blot force 5) followed by vitrification in liquid ethane.
5
Cryo-EM Single Particle Sample Prep
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For the single particle analyses, 4 µL of the samples at a protein concentration of 1.5 µg/µl was applied onto Quantifoil R2/2 300-mesh holey carbon grid, coated with thin home-made continuous carbon film and glow-discharged (2.5 mA for 20 sec). The sample was incubated on the grid for 30 sec and then blotted with filter paper for 2 sec in a temperature and humidity controlled Vitrobot Mark IV (T = 4°C, humidity 100%, blot force 5) followed by vitrification in liquid ethane.
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