Log In Sign up
The largest database of trusted experimental protocols

Nb400 129

Manufactured by Novus Biologicals
Visit Supplier
Sourced in Canada

The NB400-129 is a laboratory equipment product offered by Novus Biologicals. It is designed for a specific core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation on the intended use.

Automatically generated - may contain errors

Lab products found in correlation

Ab24170, by Abcam (3 mentions) L0668, by Merck Group (2 mentions) Ab292, by Abcam (1 mentions) α smooth muscle actin α sma clone 1a4, by Merck Group (1 mentions) Bond dewax solution, by Leica (1 mentions) Bond epitope retrieval solution 1, by Leica (1 mentions) Bs 0561r, by Bioss Antibodies (1 mentions) Alexa fluor 488 647, by Thermo Fisher Scientific (1 mentions) Protease inhibitor, by Roche (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Clt9002, by Cedarlane (1 mentions) Af2330, by R&D Systems (1 mentions) Mab9521, by R&D Systems (1 mentions) Sc 2020, by Santa Cruz Biotechnology (1 mentions) Haf005, by R&D Systems (1 mentions) Sc 46657, by Santa Cruz Biotechnology (1 mentions)

4 protocols using nb400 129

1

Immunohistochemical Profiling of Lysosomal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used Abs against the C-terminal region of rat and mouse LAMP-1 (ab24170; Abcam, Cambridge, MA), LAMP-2 (L0668; Sigma-Aldrich, St. Louis, MO), LAMP-2a (51-2200; Invitrogen/Life Technologies, Carlsbad, CA), and lysosomal integral membrane protein type-2 (LIMP-2) (NB400-129; Novus Biologicals Oakville, ON, Canada); and against full-length/luminal mouse LAMP-1 (1d4b; Iowa Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA), and mouse LAMP-2 (GL2A7; Iowa Developmental Studies Hybridoma Bank). The following Abs were used for immunofluorescence and immunohistochemical analyses: collagen-1 (ab292; Abcam), Ki67 (IHC-00375; Bethyl Laboratories, Inc. Montgomery, TX), α-smooth muscle actin (α-SMA) (clone 1A4; Sigma-Aldrich), insulin (4590; Cell Signaling Technology, Beverly, MA), CD68 (ABIN181836; Antibodies Online, Atlanta, GA), and CD206 (OASA05048; Aviva Systems Biology, San Diego, CA).
Mareninova O.A., Sendler M., Malla S.R., Yakubov I., French S.W., Tokhtaeva E., Vagin O., Oorschot V., Lüllmann-Rauch R., Blanz J., Dawson D., Klumperman J., Lerch M.M., Mayerle J., Gukovsky I, & Gukovskaya A.S. (2015). Lysosome-Associated Membrane Proteins (LAMP) Maintain Pancreatic Acinar Cell Homeostasis: LAMP-2–Deficient Mice Develop Pancreatitis. Cellular and Molecular Gastroenterology and Hepatology, 1(6), 678-694.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of EV-A71, PSGL1, and SCARB2

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin fixed, paraffin embedded tissue sections were deparaffinized using Bond Dewax Solution (Leica) after which Epitopes were retrieved using Bond Epitope Retrieval Solution 1 (Leica). The sections were stained for EV-A71 antigen (10F0, abcam), PSGL1 (bs-0561R, Bioss) or SCARB2 (NB400-129, Novus). In addition, haematoxylin and eosin staining was performed to visualize inflammation.
Sun L., Tijsma A., Mirabelli C., Baggen J., Wahedi M., Franco D., De Palma A., Leyssen P., Verbeken E., van Kuppeveld F.J., Neyts J, & Thibaut H.J. (2019). Intra-host emergence of an enterovirus A71 variant with enhanced PSGL1 usage and neurovirulence. Emerging Microbes & Infections, 8(1), 1076-1085.
+ Open protocol
+ Expand
3

Quantitative Proteomic Analysis of Cellular Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen tissue samples and cultured cells were lysed in KPi lysis buffer (25 mM K2HPO4/KH2PO4, pH 6.5, 0.1% (v:v) Triton X-100) supplemented with protease inhibitors (Roche) and sonicated 5× for 1 s with 9 min intervals (amplitude 25%). Equal quantities of protein as assessed by bicinchoninic acid assay (Thermo Fisher Scientific, 23225) were resuspended in Laemmli buffer and denatured at 95 °C. Proteins were subsequently separated by SDS-PAGE using a 10% acrylamide gel and transferred to 0.2 µm nitrocellulose membrane (#1704159, Bio-Rad). Blocking of membranes occurred in 5% (w:v) bovine serum albumin (Sigma, A1906) solution in PBS/0.1% Tween-20 (Sigma, P1379) for 1 h at room temperature (RT). Primary antibodies used were targeted against GPNMB (AF2330; R&D Systems), LIMP2 (NB400-129, Novus Biologicals), and galectin-3 (MABT51, Millipore). Furthermore, rabbit-anti-LAMP1 was used (ab24170, Abcam) and mouse-anti-tubulin (Cedarlane, CLT 9002, Burlington, ON, Canada) was used as loading control. Proteins were detected by using specific secondary conjugated antibodies (Alexa FluorTM 488/647) (Molecular Probes). Detection of immunoblots was performed using a Typhoon FLA 9500 fluorescence scanner (GE Healthcare).
van der Lienden M.J., Aten J., Marques A.R., Waas I.S., Larsen P.W., Claessen N., van der Wel N.N., Ottenhoff R., van Eijk M, & Aerts J.M. (2021). GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice. International Journal of Molecular Sciences, 22(5), 2532.
+ Open protocol
+ Expand
4

Immunoblot and Immunofluorescence Antibody Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for immunoblot analysis and immunofluorescence labeling: anti-cathepsin C (dilution: 1:1000, sc-5647, Santa Cruz Biotechnology, RRID: AB_2086961), anti-trypsin (dilution: 1:1000, sc-137077, Santa Cruz Biotechnology, RRID: AB_2300318), anti-amylase (dilution: 1:1000, sc-46657, Santa Cruz Biotechnology, RRID: AB_626668), anti-cathepsin B (dilution: 1:1000, MAB965, R&D systems, RRID: AB_2086935), anti-cathepsin L (dilution: 1:1000, MAB9521, R&D systems, RRID: AB_2087829), anti-α/β-tubulin (dilution: 1:1000, #2148, Cell Signaling, RRID: AB_2288042), anti-Rab7 (dilution: 1:1000 (for Immunoblot) and 1:100 (for immunofluorescence), #9367, Cell Signaling, RRID: AB_1904103), anti-syncollin (dilution: 1:1000, ab178415, Abcam), anti-LAMPI (dilution: 1:1000, ab24170, Abcam, RRID: AB_775978), anti-GAPDH (dilution: 1:1000, H86504M, Meridian Life Science, RRID: AB_151542), anti-LIMPII (dilution: 1:1000, NB400-129, Novus Biologicals, RRID: AB_2301298), anti-LAMPII (dilution: 1:1000, L0668, Sigma Aldrich, RRID: AB_477154), anti-rat (dilution: 1:16,000, HAF005, R&D systems, RRID: AB_1512258), anti-goat (dilution: 1:16,000, sc-2020, Santa Cruz Biotechnology, RRID: AB_631728), anti-rabbit (dilution: 1:16,000 (for immunoblots) or 1:200 (for immunofluorescence), NA934V, Amersham) and anti-mouse (dilution: 1:16,000, NA931V, Amersham).
Zierke L., John D., Gischke M., Tran Q.T., Sendler M., Weiss F.U., Bornscheuer U.T., Ritter C., Lerch M.M, & Aghdassi A.A. (2024). Initiation of acute pancreatitis in mice is independent of fusion between lysosomes and zymogen granules. Cellular and Molecular Life Sciences: CMLS, 81(1), 207.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!