Phospho fak1

Manufactured by Cell Signaling Technology

Phospho-FAK1 is a laboratory reagent used to detect and quantify the phosphorylation state of the Focal Adhesion Kinase 1 (FAK1) protein. FAK1 is an important signaling molecule involved in various cellular processes, and its phosphorylation status is a key indicator of its activity. The Phospho-FAK1 product provides a reliable tool for researchers to analyze the phosphorylation levels of FAK1 in their experimental systems.

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Lab products found in correlation

2 protocols using phospho fak1

Comprehensive Cell Lysis and Immunoblotting

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Cells were lyzed using 0.20% NP40, 50 mM Tris pH 7.5, 5% Glycerol, 1.5 mM MgCl₂, 100 mM NaCl lysis buffer containing Phosphatase Inhibitor Cocktail 2 (Sigma, P5726) and cOmplete Protease Inhibitor Cocktail (Roche, 11873580001). Lysates were resolved by SDS-PAGE and incubated with primary antibodies. Antibodies used were against actin (A5441), CAMKK2 (HPA017389) (both Sigma), ERK1/2 (Sigma, M5670) and phospho-AKT (Ser473)(#9271), AKT (#9272), ALK(#3633), phospho-p90RSK (Ser380)(#12032), RSK1/2/3 (#9355), RSK1(#9333), RSK2 (#5528), phospho-RPS6 (Ser235/236)(#4858), RPS6 (#2217), phospho-ERK1/2 (Thr202/Tyr204)(#4267), phospho-FAK1 (Tyr397)(#8556), FAK1 (#13009), phospho-IGF1R (Tyr1131)(#3021), IGF1R (#9750), cleaved Caspase 3 (#9661), PARP-1 (#9542), AMPK1 (#2795), pAMPKα (Thr172)(#2535), FER (#4268), phospho-YB1 (Ser102)(#2900), and YB1 (#4202) were from Cell Signaling. Secondary antibodies were HRP-conjugated α-rabbit or α-mouse (GE Healthcare).

Kuenzi B.M., Remsing Rix L.L., Stewart P.A., Fang B., Kinose F., Bryant A.T., Boyle T.A., Koomen J.M., Haura E.B, & Rix U. (2017). Polypharmacology-based ceritinib repurposing using integrated functional proteomics. Nature chemical biology, 13(12), 1222-1231.

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Profiling Signaling Pathways in Cell Lines

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All human cell lines were obtained from the American Type Culture Collection (ATCC), which performs authentication on its own cell lines. All the cell lines were cultured in DMEM supplemented with 10% heat-inactivated FBS at 37°C and 5% CO₂ in a humidified incubator. Antibodies against total PDCD4 (CAT#:9535S), AKT1 (CAT#:75692), AKT2 (CAT#:3063), AKT3 (CAT#:14982), P65 (CAT#:8242), P50 (CAT#:3035), c-Rel (CAT#:4727), RelB (CAT#:10544), Tubulin (CAT#:2148s), Histone3 (CAT#:4499), Actin (CAT#:3700), STAT3 (CAT#:9139), ERK(CAT#:4695), FAK1 (CAT#:3285), phospho-STAT3 (CAT#:9145), phospho-ERK (CAT#:4370), and phospho-FAK1 (CAT#:8556) were purchased from Cell Signaling Technology. Antibody against VEGF (CAT#:ab52917) was purchased from Abcam. Human Cytokine Antibody Array C3 was purchased from Raybio.

Pin G., Huanting L., Chengzhan Z., Xinjuan K., Yugong F., Wei L., Shifang L., Zhaojian L., Kun H., Weicheng Y., Yingying L., Yongming Q, & Yanan Y. (2020). Down-Regulation of PDCD4 Promotes Proliferation, Angiogenesis and Tumorigenesis in Glioma Cells. Frontiers in Cell and Developmental Biology, 8, 593685.

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