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8 protocols using l lysine

1

Analysis of α-Dicarbonyl Compounds

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Glucose, disodium hydrogen phosphate, sodium dihydrogen phosphate, methanol, and ammonia (all of analytical grade) were purchased from Xilong Scientific Co., Ltd. (Guangdong, China). L-lysine (analytical grade) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Trifluoroacetic acid and acetonitrile (all of chromatographic grade) were purchased from J&K Scientific Ltd. (Beijing, China). Pyrraline (standard) was purchased from Beijing Pinellia Technology Development Co., Ltd. (Beijing, China). The analytical-grade standards of α-dicarbonyl compounds of 3-DG, GO, and MGO were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands).
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2

Enzymatic Assay for β-Galactosidase

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l-lysine was supplied by Sinopharm Chemical Reagent Co.,Ltd (Tianjin, China), and 3-morpholinopropanesulfoinc acid (MOPS) was supplied by Amresco (USA). The chemicals o-nitrophenol-β-d-galactopyranoside (ONPG) and o-nitrophenol were supplied by Solarbio (Beijing, China). Other chemicals used in this study were of analytical grade or better. Restriction endonucleases were purchased from Fermentas (USA). DNA polymerase was obtained from Transgene (Beijing, China). T4 DNA ligase was purchased from New England Biolabs, Inc. (Beijing, China).
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3

DNA Cloning Reagents and Enzymes

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l-Lysine and arabinose were supplied by Sinopharm Chemical Reagent Co., Ltd (Tianjin, China). Propidium iodide (PI) was purchased from Sigma-Aldrich (USA). Ampicillin and kanamycin were supplied by Solarbio (Beijing, China), and 3-morpholinopropanesulfoinc acid (MOPS) was supplied by Amresco (USA). Other pure chemicals used in this study were of analytical grade or better. DNA polymerase was obtained from Transgene (Beijing, China). The ClonExpress II One Step Cloning kit was purchased from Vazyme (Nanjing, China). The Wizard Genomic DNA Purification Kit was obtained from Promega. Restriction endonucleases, T4 DNA kinase and T4 DNA ligase were purchased from New England Biolabs, Inc. (Beijing, China).
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4

Protein Extraction and Analysis

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Commercial WG flour was purchased from Anhui Ante Food Co., Ltd (Suzhou, Anhui, China) with a protein content of 77.35 ± 0.97% (The total protein contents were determined with a Kjeltec8400 (FOSS analytical instruments Co. Ltd, Denmark)) on a dry matter basis and 7.27 ± 0.04% moisture. l-Lysine was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Page Ruler Prestained Protein Ladder was purchased from Thermo Scientific (MA, USA). 5, 5-dimercapto-2, 2-dinitrobenzoic acid (DTNB) was purchased from Yuanye Biotechnology Co., Ltd (Shanghai, China). The 8-Anilino-1-naphthalenesulfonic acid hydrate ammonium salt (ANS) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All the other chemical reagents were of analytical grade unless otherwise specified.
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5

Salted Sea Cucumber Processing

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Salted sea cucumbers (Apostichopus japonicus) were purchased from Nanshan fish market (Qingdao, China), and stored in laboratory −18 °C refrigerator.
All sugars were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China); Sodium periodate, methanol, ethyl acetate, hydroxylamine hydrochloride, sodium hydroxide, acetic acid, glutaraldehyde, ethanol, o-phthaldialdehyde, SDS, β-mercaptoethanol, Sodium tetraborate, L-lysine etc. were all purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China); Dialdehyde starch was purchased from Taian Jinshan Modified Starch Co., Ltd. (Shandong, China).
All reagents were of analytical grade or the best grade available.
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6

EVOH-CAB Polymer Synthesis and Modification

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Poly(ethylene-co-vinyl alcohol) (EVOH, model: ET3803, 38 mol% ethylene) was purchased from Nippon Gohsei, Japan. Cellulose acetate butyrate ester (CAB, model: 381-20) was supplied by the Eastman Chemical. 1,4-Dioxane, cyanuric chloride, l-lysine, epichlorohydrin, diethylenetriamine and potassium dichromate (K2Cr2O7), were supplied by Sinopharm Chemical Reagent Co., Ltd.
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7

Electrochemical Sensor Fabrication and Testing

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Polyacrylonitrile (PAN) (molecular weight (Mw) = 150,000), sodium hydroxide (NaOH), cupric acetate monohydrate (Cu(CH3COO)2·H2O), sodium chloride (NaCl), cobalt(II) acetate tetrahydrate (Co(CH3COO)2·4H2O), d-glucose, ascorbic acid (AA), uric acid (UA), l-lysine, dopamine (DA), and N,N-dimethylformamide (DMF) were provided by Sinopharm Chemical Reagent Corporation (China). Nafion (5 wt.%) was obtained from Sigma-Aldrich. Thiophene, thiophene-3-boronic acid monolayer acid (TBA), and sodium fluoride (NaF) were purchased from Macklin Chemical Reagent Corporation (Shanghai, China).
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8

Bevacizumab Formulation Development

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Bevacizumab (Avastin®) was purchased from Genentech/Roche (San Francisco, CA, USA). Polyvinyl alcohol (PVA 22000), human serum albumin (HSA), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Egg yolk phospholipids (EPC), soybean phospholipids (SPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) were purchased from Lipoid GmbH (Ludwigshafen, Germany). Triolein, cholesterol, L-lysine, sucrose, glucose, and other materials were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). VEGF165 and goat anti-human IgG (Fc-specific) secondary antibody (horseradish peroxidase [HRP]) were purchased from Beijing Sino Biological Inc. (Beijing, China).
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