Crc 25w

Manufactured by Mirion Technologies

The CRC-25W is a portable, battery-operated radiation survey meter designed for the measurement of ambient gamma radiation levels. It features a Geiger-Müller tube detector and provides digital readouts of radiation dose rate and cumulative dose. The device is compact, lightweight, and offers basic functionality for radiation monitoring purposes.

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Lab products found in correlation

Automatic gamma counter, by Hidex (1 mentions) Pd minitrap g 25, by GE Healthcare (1 mentions) Vivaspin turbo 4, by Sartorius (1 mentions)

5 protocols using crc 25w

Comprehensive Tissue Radioactivity Analysis

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After imaging, mice were euthanized by cervical dislocation under isoflurane anesthesia. Blood, bone, brain, heart, large intestine, kidneys, liver, lungs, muscle, pancreas, skin, spleen, stomach, and tumors were resected, weighed, and had their activity measured by a CRC-25W (Capintec) gamma counter averaged over 45 seconds. The CRC-25W collected counts from all windows and was calibrated through serial dilutions based on the dose reported by the Atomlab 100 dosimeter used to measure injected dose. Renal radiation dose was calculated with the Medical Internal Radiation dose method.

Kruziki M.A., Case B.A., Chan J.Y., Zudock E.J., Woldring D.R., Yee D, & Hackel B.J. (2016). A 64Cu-labeled Gp2 Domain for PET Imaging of Epidermal Growth Factor Receptor. Molecular pharmaceutics, 13(11), 3747-3755.

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Tissue Harvesting and Radioactivity Quantification

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After imaging, mice were euthanized by cervical dislocation while still under isoflurane anesthesia. After verifying death via toe pinch, mice were dissected and blood, heart, lung, liver, pancreas, spleen, stomach, intestine, skin, A431 tumor, MDA-MB-435 tumor, brain, bone, muscle, and kidneys were collected and weighed. Sample radioactivity was measured by either a CRC 25W (Capintec, Florham Park, NJ) gamma counter over all energy ranges or an Automatic Gamma Counter (Hidex, Turku, Finland) between 400 and 600 keV with all reads averaged over 60 s. Counts from both instruments were calibrated using serial dilutions based on the dose reported by the Atom Lab 100 dosimeter.

Case B.A., Kruziki M.A., Stern L.A, & Hackel B.J. (2017). Evaluation of affibody charge modification identified by synthetic consensus design in molecular PET imaging of epidermal growth factor receptor. Molecular systems design & engineering, 3(1), 171-182.

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Biodistribution of 64Cu-labeled GαE Proteins

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Mice were anesthetized with 2% isoflurane at 1 mL min⁻¹ oxygen until stationary. When no longer ambulatory, 1.0–1.9 MBq ⁶⁴Cu-NODAGA-GαE22, -GαE35, or -GαE57 was injected via lateral tail vein. At 2 hours p.i., mice were again anesthetized then euthanized by cervical dislocation. After confirming expiration by toe pinch, mice were dissected and blood, heart, lung, liver, pancreas, spleen, stomach, intestine, skin, brain, bone, muscle, kidney, A431, MDA-MB-435 were extracted and weighed. Resected samples were measured for radioactivity by a CRC-25W (Capintec, Florham Park, NJ) gamma counter across all energy ranges and averaged over 1–2 min. Incident counts were calibrated using serial dilutions based on the dose reported by an Atom Lab dosimeter.

Case B.A., Kruziki M.A., Johnson S.M, & Hackel B.J. (2018). Engineered charge redistribution of Gp2 proteins through guided diversity for improved PET imaging of epidermal growth factor receptor. Bioconjugate chemistry, 29(5), 1646-1658.

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Radioiodination of ST03-Cupid Protein

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Example 5

Using N-succinimidyl-3-(4-hydroxy-3-[¹²⁵I]iodophenyl)propionate, Bolton-Hunter Reagent (NEX120, PerkinElmer), the purified ST03-Cupid protein was radio-iodinated. Specifically, Bolton-Hunter Reagent in an amount of 20 times the number of moles of the used protein was taken in an Eppendorf tube, the solvent was then vaporized, and the protein solution was then added thereto. The mixture was reacted on ice for 2 hours. Thereafter, the reaction mixture was subjected to a desalination column (PD MiniTrap G-25, GE Healthcare) to remove unreacted radio-iodination Bolton-Hunter Reagent, and the resultant was then subjected to an ultrafiltration column (VIVASPIN Turbo 4, Sartorius) for concentration. After completion of the concentration, the amount of radioactivity was measured using a dose calibrator (CRC-25w, CAPINTEC), and the quality of the protein was checked by performing CBB staining according to SDS-PAGE.

US11866457B2. Clearing agent (2024-01-09). SAVID THERAPEUTICS INC. [JP]. Inventors: Masanobu Tsukagoshi [JP].

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Quantifying PET/CT Small-Animal Imaging

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We hypothesized that scanning the length of the sample block in whole body mode and imaging a syringe in a single bed position would yield estimates of total sample and syringe activities that were in close agreement with those obtained directly by well counting these same samples (the “gold standard”) and by comparison to dose calibrator measurements of syringe activities. We tested this hypothesis as described below.
A dose calibrator (CRC® 25W, Capintec, Inc., Florham Park, NJ) was used as the standard assay device for all syringe and sample measurements. The BioPET/CT [1 (link)] (Sedecal, Madrid, Spain) served as the PET/CT small-animal scanner and a 2480 Wizard 3 scintillation well counter system with automatic sample changer (Perkin Elmer, Shelton, CT) as the well counter.

Green M.V., Seidel J., Choyke P.L, & Jagoda E.M. (2017). A Simple Device to Convert a Small-Animal PET Scanner into a Multi-Sample Tissue and Injection Syringe Counter. Nuclear medicine and biology, 53, 9-13.

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