Pccl nr4a2

Manufactured by Addgene

The PCCL-NR4A2 is a lab equipment product. It is a plasmid construct that contains the NR4A2 gene.

Automatically generated - may contain errors

Lab products found in correlation

β actin sc 1616 hrp, by Santa Cruz Biotechnology (2 mentions) Pcdna p63, by Addgene (2 mentions) Trichostatin a tsa, by Merck Group (1 mentions) Suberoylanilide hydroxamic acid saha, by Merck Group (1 mentions) Dibenzazepine dbz, by Merck Group (1 mentions)

2 protocols using pccl nr4a2

Notch Signaling and Histone Acetylation Regulation

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Valproic acid sodium salt (VPA), DAPT (LY-374973, N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester), dibenzazepine (DBZ) and trichostatin A (TSA) and suberoylanilidehydroxamic acid (SAHA), were purchased from Sigma (St. Louis, MO). Antibodies of p21 (sc-756), p63 (sc-8343), COX2 (sc-7951), histone 4 (H4, sc-10810), HDAC4 (sc-11418), acetylation of histone 4 (AcH4, sc-8660-R), Notch1 (sc-23299), NR4A2 (Nurr1, N-20, sc-991), HES1 (sc-25392) and β-actin (sc-1616-HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The plasmids expressing the intracellular domains of the four Notch receptors (ICN1, ICN2, ICN3, and ICN4) were gifts from Dr. Wu (University of Florida). The plasmids with the genes NR4A2 (Nurr1) and p63 were obtained from Addgene (www.addgene.org), including pcDNA-p63 (27008), pCCL-NR4A2 (plasmid 35000) and the control pCCL (10881).

Zhu B., Sun L., Luo W., Li M., Coy D.H., Yu L, & Yu W. (2017). Activated Notch signaling augments cell growth in hepatocellular carcinoma via up-regulating the nuclear receptor NR4A2. Oncotarget, 8(14), 23289-23302.

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Notch Signaling Pathway Modulation

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The plasmids expressing the intracellular domains of the four Notch receptors (ICN, ICN2, ICN3, and ICN4) and dnMAML (DNL) were gifts from Dr. Wu (University of Florida). The plasmids with the genes NR4A2 (Nurr1), Vinculin (VCL), THBS1 (TSP1), p63 and Twist were obtained from Addgene (www.addgene.org), including pCCL-NR4A2 (plasmid 35000) and the controls pCCL (10881), pmEN1-VCL (54304), pmEN1 (53976), pBabe-Twist1 (1783), pBabe (10668), pcDNA-THBS1 (12405) and pcDNA-p63 (27008). Antibodies of p21 (Cat. No.: sc-756), p63 (sc-8343), c-Myc (sc-788), NR4A2 (sc-990), HES1 (sc-25392) and β-actin (sc-1616-HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Sun L., Liu M., Sun G.C., Yang X., Qian Q., Feng S., Mackey L.V, & Coy D.H. (2016). Notch Signaling Activation in Cervical Cancer Cells Induces Cell Growth Arrest with the Involvement of the Nuclear Receptor NR4A2. Journal of Cancer, 7(11), 1388-1395.

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