The ACC cell lines H295R and SW13 were obtained from the American Type Culture Collection (Manassas, VA, USA). They were cultured in 60 cm2 dishes at 37°C in a humidified incubator at 5% CO2. The medium for H295R were consisted of DMEM/F12 (Gibco, USA), supplemented with 2.5% Nu-serum I (Corning, USA), 1% ITS+ Premix (Corning, USA), 1% L-glutamine and 1% penicillin-streptomycin (Gibco, USA). SW13 cells were grown in DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin.
Sw 13
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SW-13 is a centrifuge designed for general laboratory applications. It features a maximum speed of 13,000 RPM and a maximum RCF of 16,060 x g. The SW-13 can accommodate a variety of sample sizes and rotor configurations to meet the needs of diverse laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Nu serum 1, by Corning (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Its premix, by Corning (2 mentions)
Penicillin streptomycin, by Solarbio (2 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Insulin transferrin selenium, by BD (1 mentions)
Nuserum, by BD (1 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by BD (1 mentions)
Dmem 10 fbs, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Merck Group (1 mentions)
Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions)
Sw 13, by Procell (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Nci h295r, by Thermo Fisher Scientific (1 mentions)
7 protocols using sw 13
1
Culture and Maintenance of ACC Cell Lines
2
Cell Culture Conditions for SW-13 and H295R
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SW-13 and H295R cell lines were both purchased from ATCC (Manassas, VA). SW-13 cells were cultured in DMEM media with 10 % Fetal Bovine Serum (FBS), 1 % Penicillin–Streptomycin and 1 % l -Glutamine (all from Gibco, Grand Island, NY). H295R cells were cultured in DMEM/F12 media with 1 % Penicillin–Streptomycin, 1 % l -Glutamine (all from Gibco, Grand Island, NY), 2.5 % NuSerum and 1 % insulin-transferrin-selenium (ITS) (both from BD Biosciences, San Jose, CA). All cell cultures were maintained in a 5 % CO2 atmosphere at 37 °C.
3
Adrenocortical Carcinoma Cell Culture
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Adrenocortical carcinoma cell line SW-13 and H295R cells were obtained from China Infrastructure of Cell Line Resource. SW-13 was cultured in Leibovitz’s L-15 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) and H295R were cultured in DMEM/F12 Medium (Gibco) supplemented with 2.5% Nu-Serum I (Corning) and ITS+ premix (Corning). Cells were cultured at 37 °C in a humidified atmosphere containing 5% CO2.
4
Culturing Human Adrenocortical Carcinoma Cells
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SW-13 and NCI-H295R human ACC cells were purchased from Shanghai Cell Bank, the Chinese Academy of Sciences (Shanghai, China). The SW-13 Cells were maintained in DMEM 10% FBS (Gibco, USA), 1 mM glutamine, and 100 U/ml penicillin-streptomycin (Solarbio, Beijing, China). The NCI-H295R cells were cultured in DMEM/F12 (Sigma-Aldrich, Japan) supplemented with 5% FBS, 100 U/ml penicillin-streptomycin and 0.1% ITS (BD Biosciences, USA).
5
Characterization of Adrenocortical Carcinoma
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Human ACC cell lines (SW13, NCI-H296R) were purchased from the Procell Life Science & Technology Co., Ltd. in Wuhan, China. The cell line was identified by the China Centre for Type Culture Collection in Wuhan, China. SW13 was incubated in DMEM medium (Gibco, China), supplemented with 10% fetal bovine serum (FBS) (Gibco, Australia) according to the ATCC instructions. NCI-H296R was grown and maintained in DMEM media supplemented with 1% insulin transferrin selenium (Gibco, China) and 2.5% Nu-Serum I (Gibco, China) in a standard humidified incubator at 37°C in a 5% CO2 atmosphere. ACC and adjacent normal adrenal tissues (n = 8) were obtained from patients undergoing laparoscopic unilateral adrenalectomy at Zhongnan Hospital of Wuhan University. Two pathologists independently confirmed the histological diagnosis. All specimens were immediately fixed in 4% PFA (paraformaldehyde) and stored in liquid nitrogen. Prior to their operation, none of the patients enrolled in the study receive any treatment. The use of these ACC specimens was approved by the Ethics Committee at Zhongnan Hospital of Wuhan University, and informed consent was obtained from all patients.
6
Culturing SW-13 and NCI-H295R Cell Lines
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SW-13 (RRID: CVCL_0542) and NCI-H295R (RRID: CVCL_0458) cell lines were purchased from Center for Excellence in Molecular Cell Science, CAS (Shanghai, China). SW-13 were maintained in L-15 medium supplemented with 10% foetal bovine serum (Gibco, USA), 1 mM glutamine, and 100 U/mL penicillin-streptomycin (Solarbio, Beijing, China). NCI-H295R were maintained in special medium for NCI-H295R cells. (Procell, Wuhan, China). The cell lines were authenticated by STR profiling.
7
Cell Culture Methodology for ACC Modeling
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Human ACC cell lines NCI-H295R (25 (link)) and SW-13 (26 (link)) and human embryonic kidney cell line HEK-293 (27 (link)) were obtained from ATCC (The ATCC Cell Biology Collection). NCI-H295R, SW-13, and HEK-293 were cultured, respectively, in RPMI medium with 2% fetal bovine serum (FBS) and 1% insulin-transferrin-selenium, L-15 medium with 10% FBS, and DMEM medium with 10% FBS (Gibco, Grand Island, NY, USA) at 37°C in a 95% air-5% CO2, in fully humidified environment. The culture used was authenticated by STR DNA profiling analysis.
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