Psg5 pparα

Manufactured by Addgene

PSG5–PPARα is a plasmid construct containing the coding sequence for the Peroxisome Proliferator-Activated Receptor alpha (PPARα) gene. PPARα is a nuclear receptor that regulates the expression of genes involved in fatty acid and lipid metabolism.

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Lab products found in correlation

Ppre x3 tk luc, by Addgene (2 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Ciprofibrate, by Cayman Chemical (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Lipo8000, by Beyotime (1 mentions) E1910, by Promega (1 mentions)

2 protocols using psg5 pparα

Evaluating PPAR Activation Pathways

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Ciprofibrate and rosiglitazone 1, were obtained from Cayman Chemical (Ann Arbor, MI). Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were purchased from Hyclone (South Logan, Utah). Penicillin/streptomycin and trypsin were procured from Gibco (Grand Island, NY). Specific plasmids pSG5–PPARα (plasmid 22751) and PPRE X3-tk-luc (plasmid 1015) were obtained from Addgene (Cambridge, MA). pCMV-rPPARγ and pPPREaP2-tk-luc were provided by Dr. Dennis Feller (Department of Pharmacology, University of Mississippi).

Yalamanchili C., Chittiboyina A.G., Haider S., Vasquez Y., Khan S., do Carmo J.M., da Silva A.A., Pinkerton M., Hall J.E., Walker L.A, & Khan I.A. (2019). In search for potential antidiabetic compounds from natural sources: docking, synthesis and biological screening of small molecules from Lycium spp. (Goji). Heliyon, 6(1), e02782.

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Evaluating PPAR-α Activation by GluCer

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We co-transfected the PPRE X3-TK-luc (1015; Addgene) and pSG5 PPAR-α (22751; Addgene) plasmid using Lipo8000 (C0533; Beyotime) into HEK293T cells according to the Lipo8000 protocol; then, we added appropriate concentrations of GluCer (131304P; Sigma-Aldrich). We used a dual-luciferase reporter assay system (E1910; Promega) and a MIX Microtiter plate luminometer (Thermo Scientific) to determine luciferase activity in cell lysates.

Zhang L., Li Y., Wang Y., Qiu Y., Mou H., Deng Y., Yao J., Xia Z., Zhang W., Zhu D., Qiu Z., Lu Z., Wang J., Yang Z., Mao G., Chen D., Sun L., Liu L, & Ju Z. (2022). mTORC2 Facilitates Liver Regeneration Through Sphingolipid-Induced PPAR-α-Fatty Acid Oxidation. Cellular and Molecular Gastroenterology and Hepatology, 14(6), 1311-1331.

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