Anti phosphorylated iκb

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-phosphorylated IκB is a laboratory reagent used to detect and quantify the phosphorylated form of the IκB protein. IκB is a regulator of the NF-κB transcription factor, and its phosphorylation is a key step in the activation of the NF-κB signaling pathway. This product enables researchers to study the activation of the NF-κB pathway in various cellular and biological systems.

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Lab products found in correlation

Anti occludin, by Thermo Fisher Scientific (1 mentions) Anti claudin 1, by Thermo Fisher Scientific (1 mentions) Anti p65, by Cell Signaling Technology (1 mentions) Anti phosphorylated p65, by Cell Signaling Technology (1 mentions) Anti tlr4, by Abcam (1 mentions) Anti iκb, by Cell Signaling Technology (1 mentions) Anti traf6, by Abcam (1 mentions) Hematoxylin, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Eosin solution, by Merck Group (1 mentions) Ab204730, by Abcam (1 mentions) Sivelestat, by Merck Group (1 mentions) Dx 4000 fitc, by Merck Group (1 mentions) Anti moma 2 antibody, by Abcam (1 mentions) Anti ly6g antibody, by BD (1 mentions) Anti gapdh antibody, by Cell Signaling Technology (1 mentions) Anti histone h3, by Cell Signaling Technology (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Anti phosphorylated nf κb p65 antibody, by Cell Signaling Technology (1 mentions) Irdye dye labeled secondary antibodies, by LI COR (1 mentions)

Spelling variants of this product

Anti-IκB (55 citations) Phosphorylated (p)-IκB (4 citations)

3 protocols using anti phosphorylated iκb

Western Blot Analysis of Tight Junction Proteins

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Primary antibodies used for Western blot analysis were anti-zonula occludens 1 (ZO-1), anti-occludin, anti-claudin-1 (Thermo Scientific, Rockford, USA), anti-phosphorylated p65, anti-phosphorylated IκB, anti-p65, anti-IκB (Cell Signaling Technology, Danvers, USA), anti-TIRAP (Toll-interleukin 1 receptor domain containing adaptor protein), anti-IRAK1 (interleukin-1 receptor-associated kinase 1), anti-IRAK4 (Proteintech, Wuhan, China), anti-TRAF6 (TNF receptor-associated factor 6), anti-TLR4 (Abcam, Cambridge, MA, USA), anti-β-actin, and anti-Histone H3 (Hua'an Biological Technology, Hangzhou, China). The antibody-antigen complexes were visualized by using the ECL (Electrochemiluminescence) kit (Millipore, Billerica, USA). The intensity of the immunoreactive bands was semiquantified using the GeneTools (SynGene, Frederick, USA).

Shu X., Wang M., Xu H., Liu Y., Huang J., Yao Z, & Zhang L. (2017). Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 9208314.

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Evaluating Intestinal Inflammation in Mice

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The Oil Red O staining kit (#MAK194), hematoxylin (#MHS32), eosin solution (#HT110116), sivelestat (#S7198), and lipopolysaccharides (#L2630) were purchased from Sigma (Sigma Chemicals, St. Louis). Anti-Ly6G antibody (#561105) was purchased from BD (BD Biosciences). Anti-moma-2 antibody (#ab33451) was purchased from Abcam (Cambridge, United Kingdom). Anti-ZO-1 (#5406), anti-Tubulin (#5568), anti-phosphorylated IκB (#2859), and anti-IκB (#9242) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). DX-4000-FITC (#46944) was purchased from Sigma (Sigma chemicals, St. Louis), and the endotoxin kit (#88282) was from Pierce (Thermo Fisher, CA). Intestinal protein levels of TNF-α (#BMS607-3), IL-1β (#BMS6002), and MCP-1 (#BMS6005) were measured by using ELISA kits from Invitrogen (Thermo Fisher, CA). The intestinal neutrophil elastase activity was measured with a commercial kit (# ab204730) purchased from Abcam (Cambridge, United Kingdom).

Nie H., Xiong Q., Lan G., Song C., Yu X., Chen L., Wang D., Ren T., Chen Z., Liu X, & Zhou Y. (2022). Sivelestat Alleviates Atherosclerosis by Improving Intestinal Barrier Function and Reducing Endotoxemia. Frontiers in Pharmacology, 13, 838688.

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NF-κB Activation Pathway in Motoneurons

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At the indicated selected time points after exposure to ACM or infection with viral vector, MNs were harvested in lysis buffer containing sodium orthovanadate and sodium fluoride to avoid dephosphorylation. The following primary antibodies were used: rabbit anti-total NF-κB p65 antibody (Cell Signaling), anti-phosphorylated NF-κB p65 antibody (Cell Signaling), anti-GAPDH antibody (Cell Signaling), anti-histone H3 (Cell Signaling), anti-total IκB (Cell Signaling), anti-phosphorylated IκB (Cell Signaling), and mouse anti- β-actin (Sigma). The dilution used for all the antibodies was 1:1000, except for β-actin, for which it was 1:20,000. Western blots using antibodies raised against the cytosolic and nuclear markers cited above were used as loading controls. All bands were quantified by Odyssey Infrared Imaging system using IRDye dye-labeled secondary antibodies (LI-COR). Results were expressed as ratios of arbitrary fluorescence units for the protein of interest over arbitrary fluorescence units for loading controls or as ratios of phosphorylated protein of interest over total protein of interest.

Ikiz B., Alvarez M.J., Ré D.B., Le Verche V., Politi K., Lotti F., Phani S., Pradhan R., Yu C., Croft G.F., Jacquier A., Henderson C.E., Califano A, & Przedborski S. (2015). The regulatory machinery of neurodegeneration in in vitro models of amyotrophic lateral sclerosis. Cell reports, 12(2), 335-345.

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