Mpeg2000 dspe

Manufactured by Lipoid

Sourced in Germany

MPEG2000-DSPE is a lipid-based excipient used in the formulation of pharmaceutical and cosmetic products. It serves as a stabilizing agent, enhancing the physical and chemical stability of the final product.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Cremophor el, by BASF (2 mentions) Sephadex g 50, by Merck Group (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) 5 carboxyfluorescein fam, by Merck Group (1 mentions) Fmoc protected amino acids, by GL Biochem (1 mentions) Doxorubicin hydrochloride, by Meilun (1 mentions) 4 1 1 3 3 tetramethylbutyl phenyl polyethylene glycol triton x100, by Merck Group (1 mentions) Tris hydroxymethyl aminomethane tris, by Merck Group (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir, by Thermo Fisher Scientific (1 mentions) Egg phosphatidylcholine epc, by Lipoid (1 mentions) Phosphate buffered saline pbs ph, by Merck Group (1 mentions) Cholesterol, by Sinopharm (1 mentions) Huvecs, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions) Ebm 2, by Lonza (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions) Hepes buffer, by Merck Group (1 mentions) Silibinin, by Merck Group (1 mentions) True nuclear transcription factor buffer set, by BioLegend (1 mentions) Dnase type 1, by Roche (1 mentions)

Spelling variants of this product

Methoxypolyethyleneglycol (Mw 2000)-distearylphosphatidylethanolamine (mPEG2000-DSPE) (3 citations) 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (mPEG2000-DSPE) (3 citations)

14 protocols using mpeg2000 dspe

Glioblastoma Treatment Protocol with Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fmoc-protected amino acids were purchased from GL Biochem Ltd (Shanghai, China). HSPC (hydrogenated soy phosphatidylcholine) and mPEG₂₀₀₀-DSPE were obtained from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol was supplied by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Near infrared dye DiR was obtained from Invitrogen, USA. Sephadex G50 and 5-carboxyfluorescein (FAM) were provided by Sigma (St. Louis, MO). Doxorubicin hydrochloride was supplied by Dalian Meilun Biotech Co., Ltd (Dalian, China). Rat tail collagen Type I was purchased from Shengyou Biological Technology Co. (Hangzhou, China). DNase I and collagenase were supplied by Dingguo Biological Technology Co. Ltd (Shanghai, China).
Human glioblastoma cells (U87), human umbilical vascular endothelial cells (HUVECs) and brain capillary endothelial cells (bEnd.3) were obtained from Shanghai Institute of Cell Biology, cultured in special Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco). ICR mice and BALB/c nude mice aged 4-6 weeks were supplied by Shanghai SLAC Laboratory Animal Co. Ltd (Shanghai, China) and housed under SPF conditions. All animal experiments were carried out in accordance with the guidelines evaluated and approved by the Ethics Committee of Fudan University.

Belhadj Z., Zhan C., Ying M., Wei X., Xie C., Yan Z, & Lu W. (2017). Multifunctional targeted liposomal drug delivery for efficient glioblastoma treatment. Oncotarget, 8(40), 66889-66900.

+ Open protocol

+ Expand

Liposome Formulations for Biomedicine

Check if the same lab product or an alternative is used in the 5 most similar protocols

All phospholipids, including egg phosphatidylcholine (EPC), cholesterol (chol), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (ammonium salt) (mPEG2000-DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-3400] (ammonium salt) (MalPEG₃₄₀₀-DSPE), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD-DOPE) were commercially acquired from Lipoid GmbH (Ludwigshafen, Germany) and Avanti Polar Lipids (Alabama, USA). The detergent Tris (hydroxymethyl)-aminomethane (Tris) and 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol (Triton-X100) was purchased from Sigma (Taufkirchen, Germany), and the near infrared fluorescent (NIRF) dye, DY-676-COOH was acquired from DYOMICs GmbH (Jena, Germany).

Tansi F.L., Rüger R., Kollmeier A.M., Rabenhold M., Steiniger F., Kontermann R.E., Teichgräber U.K., Fahr A, & Hilger I. (2020). Targeting the Tumor Microenvironment with Fluorescence-Activatable Bispecific Endoglin/Fibroblast Activation Protein Targeting Liposomes. Pharmaceutics, 12(4), 370.

+ Open protocol

+ Expand

Lipid Formulation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The synthetic 1,2-dioleoyl-sn-glycero-3-phospho-l-serine sodium salt (DOPS), 1,2-dioleoyl-sn-glycero-3-phospho-1′-rac-glycerol sodium salt (DOPG), S100 (a purified natural phosphatidylcholine (PC) derived from soybean) and the PEGylated PL 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] sodium salt (MPEG-2000-DSPE) were kindly provided by Lipoid GmbH (Ludwigshafen, Germany). Chemical structures of the PLs are presented in the supplements (Figure S1). The fluorescent dye, 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindotricarbocyanine Iodide (DiR), was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). All other substances are named in the corresponding sections.

Klein M.E., Rieckmann M., Sedding D., Hause G., Meister A., Mäder K, & Lucas H. (2021). Towards the Development of Long Circulating Phosphatidylserine (PS)- and Phosphatidylglycerol (PG)-Enriched Anti-Inflammatory Liposomes: Is PEGylation Effective?. Pharmaceutics, 13(2), 282.

+ Open protocol

+ Expand

Doxorubicin Liposome Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

DOX hydrochloride (DOX-HCl) was obtained from Ebewe Pharma (Austria). Egg phosphatidylcholine (EPC), and derivatized distearyl phosphatidylethanolamine (mPEG2000- DSPE) were obtained from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol (CHOL), fluorescent label (Dil), and phosphate-buffered saline (PBS, pH=7.0) were purchased from Sigma- Aldrich Co. (St. Louis, MO, USA). Dialysis bags (MWCO 12000-14000) were supplied by Jingkehongda Biotechnology Co., Ltd. (Beijing, China). Chloroform, ethanol and other chemicals used in this study were analytical grade.

Haghiralsadat F., Amoabediny G., Sheikhha M.H., Forouzanfar T., Helder M.N, & Zandieh-doulabi B. (2017). A Novel Approach on Drug Delivery: Investigation of A New Nano-Formulation of Liposomal Doxorubicin and Biological Evaluation of Entrapped Doxorubicin on Various Osteosarcoma Cell Lines. Cell Journal (Yakhteh), 19(Suppl 1), 55-65.

+ Open protocol

+ Expand

Labeling Cells and Mice with Fluorescent Dyes

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorophores DiO, DiI and DiD were purchased from Invitrogen (Grand Island, NY, USA). 4′,6-Diamidino-2-phenylindole (DAPI) was from Roche (Basel, Switzerland). mPEG₂₀₀₀–DSPE, HSPC (hydrogenated soy phosphatidylcholine) and POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) were supplied by Lipoid GmbH (Ludwigshafen, Germany). Cholesterol was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Mal–PEG₃₄₀₀–DSPE was provided by Laysan Bio Co. (Arab, AL, USA). EBM-2 was from Lonza (Visp, Switzerland).
U87 (human glioblastoma cells) and HUVECs (human umbilical vein endothelial cells) were provided by Shanghai Institute of Cell Biology. Both cell lines were cultured in Dulbecco's modified Eagle medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS, Gibco) at 37 °C in a humidified atmosphere containing 5% CO₂. Male ICR mice and BALB/c nude mice of 6–8 week age were supplied by Shanghai SLAC laboratory animal Co., Ltd. (Shanghai, China). All animal experiments were performed in accordance with guidelines approved by the ethics committee of Fudan University, Shanghai, China.

Dai T., Jiang K, & Lu W. (2018). Liposomes and lipid disks traverse the BBB and BBTB as intact forms as revealed by two-step Förster resonance energy transfer imaging. Acta Pharmaceutica Sinica. B, 8(2), 261-271.

+ Open protocol

+ Expand

Silibinin-Loaded Stealth Liposomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dipalmitoylphosphatidylcholine (DPPC) and
methoxy-polyethylene glycol (PEG2000)-derivatized
distearoyl phosphatidylethanolamine (mPEG2000-DSPE) were obtained from Lipoid GmbH (Ludwigshafen, Germany). CHOL, silibinin and HEPES
buffer (10 mM, pH=5.5) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glycyrrhizic acid
was purchased from Shirin Darou co. (Shiraz, Iran).
Deionized water was used throughout the experiments. The in vitro release measurement was
carried out at pH=7.4 in phosphate buffer and at
pH=5.5 in HEPES buffer at 37˚C. Maltose, etha-
nol and the remainder of chemicals used in this
study were analytical grade quality. HepG2 and
fibroblast cell lines were supplied by the Pasteur
Institute of Iran.

Ochi M.M., Amoabediny G., Rezayat S.M., Akbarzadeh A, & Ebrahimi B. (2016). In Vitro Co-Delivery Evaluation of Novel Pegylated Nano-Liposomal Herbal Drugs of Silibinin and Glycyrrhizic Acid (Nano-Phytosome) to Hepatocellular Carcinoma Cells. Cell Journal (Yakhteh), 18(2), 135-148.

+ Open protocol

+ Expand

Immune Checkpoint Blockade Antibody Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hydrogenated soya phosphatidylcholine (HSPC) and Methoxypolyetheleneglycol (Mw 2000) distearylphosphatidylethanolamine (mPEG2000-DSPE) were purchased from Lipoid (Ludwigshafen, Germany). Cholesterol was purchased from Sigma–Aldrich (St. Louis, MO). Commercially available Caelyx^® was purchased from the Behestan Darou Company (Tehran, Iran). The IgG anti-mouse CTLA-4 blockade monoclonal antibody (Clone: 9H10) was purchased from Bioxcell (NH, USA). Pe-Cy5-labeled Rat anti-mouse CD4 antibody, PE-labeled Rat anti-mouse CD8a antibody, PE-labeled Rat anti-mouse CD25 antibody, Alexa-flour labeled Rat anti-mouse Foxp3 antibody, APC labeled anti-mouse CD 45 antibody, NIR Zombie die (for live/Dead discrimination), as well as appropriate isotype control antibodies and a True-Nuclear™ transcription factor buffer set were purchased from Biolegend (CA, San Diego). Collagenase type I, RPMI 1560 medium, and Fetal Bovine Serum (FBS) was purchased from Gibco (NY, USA). Dnase type I was purchased from Roche (USA). The remaining chemical solvents and reagents were chemical grade.

Alimohammadi R., Alibeigi R., Nikpoor A.R., Chalbatani G.M., Webster T.J., Jaafari M.R, & Jalali S.A. (2020). Encapsulated Checkpoint Blocker Before Chemotherapy: The Optimal Sequence of Anti-CTLA-4 and Doxil Combination Therapy. International Journal of Nanomedicine, 15, 5279-5288.

+ Open protocol

+ Expand

Optimizing PEGylated Lipid Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cholesterol, mono PEGylated 1, 2-distearoyl-glycero-3-phophoethanolamine (mPEG-2000-DSPE), and hydrogenated soybean phosphatidylcholine (HSPC) were obtained from Lipoid GmbH (Ludwigshafen, Germany). Ethanol, isopropanol, histidine, sucrose, ammonium sulfate (AS), sodium sulfate, magnesium sulfate, sodium citrate, magnesium chloride, guanidine chloride (GdmCl) were purchased from Sigma Aldrich Kft. (Budapest, Hungary). Salsol infusion (TEVA Hungary Zrt., Debrecen, Hungary) was obtained from the University Pharmacy, and purified water was produced by a Milli-Q Integral 3 Water Production Unit (Merck Millipore, Billerica, MA, USA).

Bozó T., Mészáros T., Mihály J., Bóta A., Kellermayer M.S.Z., Szebeni J, & Kálmán B. (2016). Aggregation of PEGylated liposomes driven by hydrophobic forces. Colloids and surfaces. B, Biointerfaces, 147.

+ Open protocol

+ Expand

Curcumin-Loaded Nanoparticle Formulation for Targeted Cancer Therapy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Oleic acid (OA) was obtained from Sigma-Aldrich. 2,2-Dithiodiethanol, hexanediol, and dicyclohexylcarbodiimide (DCC) were purchased from Alfa Aesar (MA). 4-dimethylaminopyridine (DMAP) and triphosgene were purchased from Adamas Reagent (China). Curcumin (CUR), CPT, DiR, Dithiothreitol (DTT), LysoTracker and AannexinV-FITC apoptosis detection kit were purchased from MEILUN Biology Technology Co., Ltd. (Dalian, China). The DSPE-mPEG₂₀₀₀ was purchased from Lipoid GmbH (Ludwigshafen, Germany). Cremophor EL was obtained from BASF (Ludwigshafen, Germany). All solvents used in this study were analytical grade. Male C57 mice (6–8 weeks old) and Sprague–Dawley rats (200–250 g) were purchased from the Laboratory Animal Center of Chengdu Medical college (Chengdu, China). All studies involving mice were approved by the institute’s animal care and use committee.

Li F., Huang Z., Chen H., Yan L., Li J., Su Y., Zhang Q., Huang Z, & Zheng Y. (2019). Redox-sensitive lipophilic prodrugs: delivering unstable chemotherapeutant for improved cancer therapy. Drug Delivery, 26(1), 1068-1079.

+ Open protocol

+ Expand

Microbial Colonization on Hernia Meshes

Check if the same lab product or an alternative is used in the 5 most similar protocols

S. aureus ATCC 6538, S. aureus ATCC 700699 (also known as MRSA Mu50) and S. epidermidis ATCC 35984 were purchased from the American Type Culture Collection (Manassas, VA, USA). Bacteria were inoculated at colony forming unit (CFU)/ml or optical density at 600 nm (OD₆₀₀) values stated after dilution of an overnight culture grown in tryptone soya broth (TSB) or nutrient broth (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C with shaking at 180 rpm. Tryptone soya agar (TSA) was prepared by adding 1.5% agar bacteriological (Thermo Fisher Scientific). The hernia meshes Parietex Hydrophilic 2-Dimensional mesh (polyester), Parietene Lightweight monofilament polypropylene mesh (polypropylene) were donated by Covidien (Dublin, Ireland). The saturated phospholipids 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycerol)-2000] (DSPE-mPEG2000) were donated by Lipoid GmbH (Ludwigshafen, Germany). Unless stated otherwise, all chemicals, materials, media and supplements were purchased from Sigma-Aldrich (Steinheim, Germany).

Kaul L., Abdo A.I., Coenye T., Swift S., Zannettino A., Süss R, & Richter K. (2023). In vitro and in vivo evaluation of diethyldithiocarbamate with copper ions and its liposomal formulation for the treatment of Staphylococcus aureus and Staphylococcus epidermidis biofilms. Biofilm, 5, 100130.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!