9100 50 emccd

Manufactured by Hamamatsu Photonics

The 9100-50 EMCCD is a high-performance electron-multiplying charge-coupled device (EMCCD) designed for low-light imaging applications. It features a 512 x 512 pixel array with 16 x 16 μm pixel size, providing high spatial resolution. The EMCCD employs an on-chip electron multiplication process to amplify the signal, enabling the detection of single photons with high sensitivity. The 9100-50 EMCCD is capable of operating at high frame rates and offers a wide range of user-selectable gain settings to suit various experimental requirements.

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Lab products found in correlation

Ultraview vox, by Nikon (2 mentions) Element software, by Nikon (2 mentions) Orca er, by Hamamatsu Photonics (2 mentions) Csu x1, by Yokogawa (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions)

2 protocols using 9100 50 emccd

Microscopic Visualization of Fluorescent Proteins

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GFP- and mCherry-fusion proteins were observed in cells after fixation with cold methanol. Cells were washed in PBS and resuspended in PBS plus 1 μg/ml DAPI (4′,6-diamidino-2-phenylindole) (Roche). Photomicrographs were obtained using a Nikon 80i fluorescence microscope coupled to a cooled CCD camera (ORCA-ER; Hamamatsu Photonics) or a Perkin Elmer spinning-disk confocal microscope (UltraVIEW^® VoX) with a 100x NA 1.49 TIRF oil immersion objective (Nikon) coupled to a cooled CCD camera (9100-50 EMCCD; Hamamatsu Photonics) and spinning disk head (CSU-X1, Yokogawa). Image processing and analysis were carried out using Element software (Nikon), ImageJ software (National Institutes of Health), and Adobe Photoshop.

Deng D.J., Xia Q.C., Jia G.S., Suo F., Chen J.L., Sun L., Wang J.Q., Wang S.M., Du L.L., Wang Y, & Jin Q.W. (2021). Perturbation of kinetochore function using GFP-binding protein in fission yeast. G3: Genes|Genomes|Genetics, 11(11), jkab290.

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Live-cell Imaging of Fluorescent Fusion Proteins

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GFP- and mCherry-fusion proteins (such as Cdc13-GFP, Cut2-GFP, Dnt1-GFP, CUEDC2-GFP, Cdc13-mCherry, and mCherry-Atb2) were observed in cells after fixation with cold methanol. Cells were washed in PBS and resuspended in PBS plus 1 μg/ml DAPI. Photomicrographs were obtained using a Nikon 80i fluorescence microscope coupled to a cooled CCD camera (Hamamatsu, ORCA-ER). Time-lapse imaging of live cells was performed at 30°C using a Perkin Elmer spinning-disk confocal microscope (UltraVIEW VoX) with a 100x NA 1.49 TIRF oil immersion objective (Nikon) coupled to a cooled CCD camera (9100–50 EMCCD; Hamamatsu Photonics) and spinning disk head (CSU-X1, Yokogawa). Image processing, analysis and spindle length measurement were carried out using Element software (Nikon), ImageJ software (National Institutes of Health) and Adobe Photoshop.

Bai S., Sun L., Wang X., Wang S.M., Luo Z.Q., Wang Y, & Jin Q.W. (2022). Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism. PLoS Genetics, 18(9), e1010397.

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