The global activities of Dnmt and Tet were determined in nuclear fractions using EpiQuik™ DNA Methyltransferase Activity/Inhibition and Epigenase™ 5mC-Hydroxylase TET Activity/Inhibition Assay Kits (EPIGENTEK) respectively. Briefly, nuclear extracts (3–6μg for Dnmt or 10–20μg for Tet activity) was added to the microplates coated with enzyme substrates, and the reaction products were detected by following the manufacturer’s instructions.
Epiquik dna methyltransferase activity inhibition
Manufactured by Epigentek
Sourced in United States
The EpiQuik™ DNA Methyltransferase Activity/Inhibition kit is a laboratory tool used to quantify the activity and inhibition of DNA methyltransferase enzymes. It provides a simple, fast, and sensitive method to measure the activity or inhibition of these enzymes in a 96-well format.
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3 protocols using epiquik dna methyltransferase activity inhibition
1
Quantifying Dnmt and Tet Activities
2
Quantifying DNMT and TET Activities
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The enzyme activities of DNMTs and TETs were quantified in the nuclear fraction (5–15 μg protein) using EpiQuik™ DNA Methyltransferase Activity/Inhi bition (#P-3001, EPIGENTEK, Farmingdale, NY, USA) and Epigenase 5mC-Hydroxylase TET Activit y/Inhibition Assay Kits (#P-3087, EPIGENTEK) in accordance with the manufacturer’s instructions.
3
Quantifying Dnmt and Tet Activities
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The global activities of Dnmt and Tet were determined in nuclear fractions using EpiQuik™ DNA Methyltransferase Activity/Inhibition and Epigenase™ 5mC-Hydroxylase TET Activity/Inhibition Assay Kits (EPIGENTEK) respectively. Briefly, nuclear extracts (3–6μg for Dnmt or 10–20μg for Tet activity) was added to the microplates coated with enzyme substrates, and the reaction products were detected by following the manufacturer’s instructions.
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