Apoptotic cells were assessed using an ApoDETECT annexin V-FITC apoptosis detection kit (Sigma-Aldrich) by flow cytometry following the kit manual. Cells treated with different concentrations of oridonin were digested, washed twice with cold PBS, and resuspended in binding buffer. Add 5 μl of FITC-labeled annexin V in 190 μl of cell suspension, mix gently, then add 5 μl propidium iodide (PI) solution. Incubate cells in dark for 10 min at room temperature. Cells were analyzed on a flow cytometer (BD Biosciences).
Apodetect annexin 5 fitc apoptosis detection kit
Manufactured by Merck Group
The ApoDETECT annexin V-FITC apoptosis detection kit is a laboratory product used to detect and analyze programmed cell death, or apoptosis, in cell cultures. The kit utilizes annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of apoptotic cells. The annexin V is conjugated with the fluorescent dye FITC, allowing for the visualization and quantification of apoptotic cells through flow cytometry or fluorescence microscopy.
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Apoptosis was assessed using an ApoDETECT annexin V-FITC apoptosis detection kit (Sigma-Aldrich) by flow cytometry following the manual. Cells were digested, washed twice with cold PBS, and re-suspended in binding buffer. FITC-labeled annexin V (5 μL) was added to the cell suspension (190μL) and mixed, followed by the addition of propidium iodide (PI) solution (5 μL). Cells were incubated in the dark at room temperature for 10 min and were then analyzed on a flow cytometer (BD Biosciences, USA).
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