Hplc grade acetonitrile ch3cn

Manufactured by Merck Group

Sourced in Germany

HPLC-grade acetonitrile (CH3CN) is a high-purity solvent used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic mild odor. HPLC-grade acetonitrile is designed to meet the strict purity requirements for HPLC analyses, ensuring consistent and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using hplc grade acetonitrile ch3cn

Electrochemical Analysis of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC-grade acetonitrile (CH₃CN) and tetrabutylammonium perchlorate
(TBAP, 98%) were purchased from Sigma-Aldrich. N₂ (99.998%,
prepurified) was gained from Cryogenic Gases. All the chemical reagents for
synthesis and analysis were analytical grade, obtained from commercial
suppliers, and used without further purification unless specified. All
electrodes for electrochemical experiments were purchased from Shanghai Chenhua
Co., Ltd., China. ¹H NMR and ¹³C NMR were acquired
in CDCl₃ on BRUKER AVANCE 500 spectrometer using TMS as an internal
standard. Mass spectrum was obtained on HP 5989 mass spectrometer.

Liu X.Y., Ma W., Zhou H., Cao X.M, & Long Y.T. (2015). Bridge- and Solvent-Mediated Intramolecular Electronic Communications in Ubiquinone-Based Biomolecular Wires. Scientific Reports, 5, 10352.

+ Open protocol

+ Expand

Quantitative Analysis of Ferulic and Isoferulic Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC-grade acetonitrile (CH3CN) and methanol were purchased from Sigma-Aldrich (St. Louis, MO, USA), while analytical grade formic acid was obtained from Huadong Chemical Reagent Co. Ltd. (Hangzhou, China). Deionized water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). Other reagents were of analytical grade.
Ferulic acid (LOT#110773-200611, purity greater than 99.0%) and isoferulic acid (LOT#11698-200602, purity greater than 99.0%) were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Antibodies against Caspase-3 (CASP3) and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). The RNA isolation kit was obtained from Bio-Rad (cat# 163-2098, Bio-Rad, Hercules, CA, USA).

Li Q., Sun J., Tu J., Li H., Zhang J., Gu H., Xie Z, & Lv H. (2022). A Promising Target of Langchuangding Prescription Treating Systemic Lupus Erythaematosus Integrated Network Pharmacology with HPLC-MS and Molecular Docking. Frontiers in bioscience (Landmark edition), 27(11).

+ Open protocol

+ Expand

HPLC Solvent Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC-grade acetonitrile (CH3CN) and formic acid were obtained from Merck KGaA (Darmstadt, Germany). HPLC-grade methanol was purchased from Sigma-Aldrich (St. Louis, MO, USA). Water was purified by the Milli-Q water purification system (Millipore, Bedford, MA, USA). All other reagent were of analytical grade.

Bao J., Liu F., Zhang C., Wang K., Jia X., Wang X., Chen M., Li P., Su H., Wang Y., Wan J.B, & He C. (2016). Anti-melanoma activity of Forsythiae Fructus aqueous extract in mice involves regulation of glycerophospholipid metabolisms by UPLC/Q-TOF MS-based metabolomics study. Scientific Reports, 6, 39415.

+ Open protocol

+ Expand

Quantitative Analysis of Epigenetic Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Water was purified using a Milli-Qpurification apparatus(Millipore, Milford, MA, USA). HPLC-grade acetonitrile (CH₃CN) was purchased from Merck KGaA (Darmstadt, Germany). Formic acid (HCOOH), ammonium formate (HCOONH₄), malic acid, oxaloacetic acid, succinic acid, citric acid, fumaric acid, α-ketoglutaric acid, phthalic acid, oxalic acid, propanedioic acid, and 5-methylcytidine (5-mrC) were purchased from Sigma-Aldrich(St Louis,MO,USA).5-Methyl-2′-deoxycytidine (5-mdC), 5-hydroxymethyl-2′-deoxycytidine (5-hmdC), 5-hydroxym ethylcytidine (5-hmrC), 5-methyl-D₃-2′-deoxycytidine ([D₃]5-mdC), 5-hydroxymethyl-D₃-2′-deoxycytidine ([D₃]5-hmdC) and 5-hydro xymethyl-¹³CD₂-cytidine ([¹³CD₂]5-hmrC) were purchased from Toronto Research Chemical (Toronto, Canada). 5-Methyl-¹³C₅-cytidine ([¹³C₅]5-mrC) was synthesized previously [22 (link)]. Stock solutions of standards and isotope-labeled internal standards (IS) were prepared at a concentration of 1 mM in water and stored at ‒ 80 °C until use.

Guo C., Xie C., Chen Q., Cao X., Guo M., Zheng S, & Wang Y. (2018). A novel malic acid-enhanced method for the analysis of 5-methyl-2′-deoxycytidine, 5-hydroxymethyl-2′-deoxycytidine, 5-methylcytidine and 5-hydroxymethylcytidine in human urine using hydrophilic interaction liquid chromatography-tandem mass spectrometry. Analytica chimica acta, 1034, 110-118.

+ Open protocol

+ Expand

Characterization of Swerilactones H-K

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC grade acetonitrile (CH₃CN) was purchased from Merck (Merck Co. Ltd., Germany). HPLC grade formic acid was purchased from Aladdin (Aladdin Chemistry Co. Ltd. China). Deionized water was purified using a MingChe™-D 24UV Merck Millipore system (Merck Millipore, Shanghai, China).
Swerilactones H–K (1–4) were isolated from S. mileensis in our previous investigation, whose structures were unambiguously determined by extensive spectroscopic data and X-ray analyses [1 (link)]. Sample solutions were prepared by dissolving each sample in a solution of 85% CH₃CN/H₂O containing 0.05% formic acid to a final concentration of 0.2 mg/mL. The samples were introduced into the source via a syringe pump at a flow rate of 2 μL/min.

Geng C.A, & Chen J.J. (2016). A Fragmentation Study on Four Unusual Secoiridoid Trimers, Swerilactones H–K, by Electrospray Tandem Mass Spectrometry. Natural Products and Bioprospecting, 6(6), 297-303.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!