Polyethylene glycol 6000 peg6000

Manufactured by Merck Group

Sourced in United States

Polyethylene glycol 6000 (PEG6000) is a synthetic, water-soluble polymer. It has a molecular weight of approximately 6,000 Daltons. PEG6000 is used as a reagent in various laboratory applications.

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Lab products found in correlation

Dotma, by Avanti Polar Lipids (1 mentions) Dntp mixture, by Takara Bio (1 mentions) Takara taq hs, by Takara Bio (1 mentions) 9 n dna ligase, by New England Biolabs (1 mentions) Pcr buffer, by Takara Bio (1 mentions) Human male genomic dna, by Promega (1 mentions) 384 well plate, by Corning (1 mentions) Sodium lauryl sulfate, by Merck Group (1 mentions) Medroxyprogesterone acetate, by Merck Group (1 mentions) Gelucire 44 14, by Gattefosse (1 mentions)

3 protocols using polyethylene glycol 6000 peg6000

Lipid Nanoparticle Delivery of Oligonucleotides

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DOTMA and DOPE were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Polyethylene glycol 6000 (PEG6000) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Epi was purchased from Pfizer, Inc. (New York, NY, USA). ASOs were obtained from Scientific Biotech Corp. (Taipei, Taiwan). All cell culture medium and reagents were purchased from Promega (Madison, WI, USA), Invitrogen (Carlsbad, CA, USA), Gibco BRL (Grand Island, NY, USA), or Hyclone (Logan, UT, USA). Most of the other chemical reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA) or Merck (Darmstadt, Germany).

Lo Y.L, & Liu Y. (2014). Reversing Multidrug Resistance in Caco-2 by Silencing MDR1, MRP1, MRP2, and BCL-2/BCL-xL Using Liposomal Antisense Oligonucleotides. PLoS ONE, 9(3), e90180.

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Oligonucleotide-based Ligation and PCR

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All DNA oligonucleotides used in this study including ligation pairs (LPs), universal primers, LNA-modified fluorescently labeled DNA probes, and synthetic targets were purchased from Integrated DNA Technologies, Inc. (Coralville, IA, USA). Reagents for ligation including 9°N DNA ligase, and 10X 9°N DNA ligase reaction buffer were purchased from New England BioLabs, Inc. (Ipswich, MA, USA, catalog #: M0238S). Polyethylene glycol-6000 (PEG-6000) as a ligation additive was purchased from Sigma-Aldrich Corp. (St. Louis, MO, catalog #: 528877). Human male genomic DNA was purchased from Promega Corp. (Madison, WI, catalog #: G1471). PCR reagents including TaKaRa Taq HS, 10X PCR buffer, and dNTP Mixture were purchased from Takara Bio USA, Inc. (Mountain View, CA, catalog #: R007A). The 384-well plate was purchased from Corning Inc. (Corning, NY, catalog #: 3540).

Park J.S., Pisanic T., Zhang Y, & Wang T.H. (2021). Ligation-enabled fluorescence-coding PCR for high-dimensional fluorescence-based nucleic acid detection. Analytical chemistry, 93(4), 2351-2358.

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Megestrol Acetate Formulation Development

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Megestrol acetate was kindly donated by LG Life Sciences (Seoul, South Korea). Hydroxypropylmethyl cellulose (HPMC; 2910) was purchased from Shin-Etsu Chemical Co Ltd (Tokyo, Japan). Polyvinylpyrrolidone (PVP; K30), PVP vinyl acetate (PVP VA64), and poloxamer 407 were kindly obtained from BASF Co Ltd (Ludwigshafen, Ger-many). D-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS; Eastman Chemical Company, Kingsport, TN, USA), Gelucire 44/14 (Gattefossé, Saint-Priest, France), and Ryoto sugar ester L1695 (Mitsubishi-Kagaku Foods Corporation, Tokyo, Japan) were used as surfactants. Medroxypro-gesterone acetate (internal standard [IS]), polyethylene glycol 6000 (PEG 6000), and sodium lauryl sulfate were purchased from Sigma-Aldrich Co Ltd (St Louis Co, MO, USA). High-performance liquid chromatography (HPLC)-grade acetonitrile, ethanol, and methanol were used in all experiments.

Ha E.S., Kim J.S., Baek I.H., Yoo J.W., Jung Y., Moon H.R, & Kim M.S. (2015). Development of megestrol acetate solid dispersion nanoparticles for enhanced oral delivery by using a supercritical antisolvent process. Drug Design, Development and Therapy, 9, 4269-4277.

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