Wtsx1

Drosophila melanogaster stocks were raised, and crosses were performed at +25°C on standard medium. The following stocks were used: elav^C155 = elav‐Gal4 (DiAntonio et al., 2001 (link)). Va‐Gal4 (Allan et al., 2003 (link)). UAS‐dac (Shen & Mardon, 1997 ). UAS‐reaper (Zhou et al., 1997 (link)). UAS‐AbdBm and FRT82B‐AbdB^D18 (obtained from E. Sanchez‐Herrero). Atu^s1938, Bgb⁹, bru3⁰⁵⁸⁷¹, dac³, dac⁴, drl^R343, Dscam1⁰⁵⁵¹⁸, dsh⁷⁵, faf^BX4, fra³, Fur1^P127, Hr38⁰²³⁰⁶, hth^5E04, kay¹, msk^B185, pan², qsm⁰⁵⁵¹⁰, rgr^k02605, rhea¹, rn⁵, run³, SoxN^NC14, stau^ry9, tou², tsh⁸, wts^x1 (Bloomington Drosophila Stock Center). Mutants were kept over CyO, Dfd‐EYFP or TM6, Sb, Tb, Dfd‐EYFP balancer chromosomes. As wild type, Oregon‐R was often used.

Stocks used in this study include the following: hsFLP; act<stop<Gal4, UAS-yki^S168A.v5, hsFLP;act<stop<Gal4, UAS-RFP^NLS, hsFLP, UAS-GFP^NLS; tub-Gal80, FRT40A (MARCM FRT 40A), ubi>RFP, FRT40A, banΔ1, banGFP, en-Gal4, dpp-lacZ, UAS-RFP, AP-1-RFP (53 (link)), and TIE-DYE (50 (link)). Stocks were obtained from the Bloomington Drosophila Stock Center (BDSC): UAS-yki^S168A.v5 (#28818), UAS-yki.v5 (#28819), UAS-yki::GFP (#28815), wts^X1 (#44251), smo³ (#3277), UAS-ci^RNAi (#64928), tara1-lacZ (#6403), UAS-tara^RNAi (#31634), nub-Gal4 (#42699), 30A-Gal4 (#37534), UAS-dMyc (#9674), UAS-rasv12 (#4847), and UAS-jnk^DN (#9311). Ci-lacZ was a gift from D. Kalderon (Columbia University, USA). UAS-bantam^sponge and UAS-bantam.D were gifts from S. Cohen (University of Copenhagen, Denmark). hs-Pc-sensor was a gift from V. Pirrotta (Rutgers University, USA). UAS-myc::tara was a gift from R. Smith-Bolton [University of Illinois, Urbana-Champaign, USA (originally from M. Cleary, University of California, Merced, USA)]. MARCM FRT19A and sd^47M, FRT19A were gifts from D. Pan.