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Genomic tip either 20 g or 100 g

Manufactured by Qiagen
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The Genomic-tip 20/G or 100/G is a laboratory equipment product manufactured by Qiagen. It is used for the purification of genomic DNA. The product functions to isolate high-molecular-weight genomic DNA from a variety of sample types.

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Lab products found in correlation

Kapa long range hotstart dna polymerase, by Roche (2 mentions)

Spelling variants of this product

Genomic-tip 20/G (71 citations) Genomic-tip (58 citations) Genomic-tips 20/G (9 citations)

2 protocols using genomic tip either 20 g or 100 g

1

Mitochondrial DNA Damage Quantification

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA isolation and quantification was performed as previously described43 (link),44 (link),68 (link) using a high molecular weight genomic DNA purification kit according to the manufacturer’s protocol (QIAGEN Genomic tip either 20/G or 100/G) and Quant-iT Picogreen dsDNA quantification. Following genomic DNA isolation, the purity and quality was assessed using a Nanodrop (ND-1000). DNA damage in the mitochondrial genome was measured utilizing the Mito DNADX the assay to calculate mitochondrial DNA lesion frequency as previously described42 (link). Reaction mixtures used KAPA Long Range HotStart DNA Polymerase (KAPABiosystems) in a 96-well platform. Primers used for human short and long amplicons can be found in69 (link). Each biological DNA sample was performed in triplicate on two independent days (for a total of 6 PCR reactions).
Sastre D., Zafar F., Torres C.A., Piper D., Kirik D., Sanders L.H., Qi S, & Schüle B. (2023). Nuclease-dead S. aureus Cas9 downregulates alpha-synuclein and reduces mtDNA damage and oxidative stress levels in patient-derived stem cell model of Parkinson’s disease. bioRxiv.
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2

Quantification and Damage Assessment of Mitochondrial DNA

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA isolation and quantification was performed as previously described43 (link),44 (link),68 (link) using a high molecular weight genomic DNA purification kit according to the manufacturer’s protocol (QIAGEN Genomic tip either 20/G or 100/G) and Quant-iT Picogreen dsDNA quantification. Following genomic DNA isolation, the purity and quality was assessed using a Nanodrop (ND-1000). DNA damage in the mitochondrial genome was measured utilizing the Mito DNADX the assay to calculate mitochondrial DNA lesion frequency as previously described42 (link). Reaction mixtures used KAPA Long Range HotStart DNA Polymerase (KAPABiosystems) in a 96-well platform. Primers used for human short and long amplicons can be found in69 (link). Each biological DNA sample was performed in triplicate on two independent days (for a total of 6 PCR reactions).
Sastre D., Zafar F., Torres C.A., Piper D., Kirik D., Sanders L.H., Qi L.S, & Schüle B. (2023). Inactive S. aureus Cas9 downregulates alpha-synuclein and reduces mtDNA damage and oxidative stress levels in human stem cell model of Parkinson’s disease. Scientific Reports, 13, 17796.
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