Single-cell suspensions from BM and spleen were treated with Gey’s solution to remove red blood cells and resuspended in PBS supplemented with 2% BSA. The cells were then stained with a combination of fluorescence-conjugated antibodies. APC-conjugated anti-B220, anti-IgM, anti-CD4 and anti-CD44, and PE-Cy7-conjugated anti-B220, anti-IFNγ, anti-CD25 and anti-CD23 were purchased from eBioscience. PE-conjugated anti-CD43, anti-CD21, anti-Thy1.2, anti-CD5, anti-CD8, and anti-IgD were purchased from BD Biosciences. Samples were applied to a flow cytometer (LSRII, Becton Dickinson). Data were collected and analyzed using FACSDiva software (Becton Dickinson) or FlowJo software (Tree Star).
Pe conjugated anti cd43
Manufactured by BD
PE-conjugated anti-CD43 is a monoclonal antibody that binds to the CD43 antigen. CD43 is a sialoglycoprotein expressed on the surface of most hematopoietic cells, including T cells, B cells, and granulocytes. The PE (Phycoerythrin) fluorescent dye is conjugated to the antibody, allowing for the detection and analysis of CD43-expressing cells using flow cytometry.
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Lab products found in correlation
Anti ifn γ, by Thermo Fisher Scientific (1 mentions)
Anti cd8, by BD (1 mentions)
Anti igd, by BD (1 mentions)
Anti cd21, by BD (1 mentions)
Anti cd5, by BD (1 mentions)
Anti thy1, by BD (1 mentions)
Anti cd23, by Thermo Fisher Scientific (1 mentions)
Pe cy7 conjugated anti b220, by Thermo Fisher Scientific (1 mentions)
Apc conjugated anti b220, by Thermo Fisher Scientific (1 mentions)
Facsdiva software, by BD (1 mentions)
Cat 15710, by Electron Microscopy Sciences (1 mentions)
Mouse igg1κ isotype control, by BD (1 mentions)
2 protocols using pe conjugated anti cd43
1
Multiparametric Flow Cytometry Analysis
2
Bone Marrow Single-Cell Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single-cell suspension from the BM of the experimental and control mice were prepared, followed by RBC lysis, and fixed with 2% PFA (Electron Microscopy Sciences Cat15710) at 37 °C for 10 min. Cells were washed twice with PBS and then slowly permeabilized with ice-cold 95% MeOH while vortexing on ice for 20 min. Cells were washed twice with PBS and incubated with Fc-block for 15 min on ice and then stained with PE/Cy7-conjugated anti-B220, PE-conjugated anti-CD43, AlexaFluor 647-conjugated anti-phospho-STAT5 (pY694, BD Biosciences), or mouse IgG1,κ isotype control (BD Biosciences). PE/Cy7-conjugated anti-B220 and PE-conjugated anti-CD43 were used at dilution of 1:400. AlexaFluor 647-conjugated anti-phospho-STAT5 was used at dilution of 1:100.
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