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Anti tnf alexafluor700

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The Anti-TNF-Alexafluor700 is a fluorescently labeled antibody that specifically binds to tumor necrosis factor-alpha (TNF-α). It is designed for use in flow cytometry and other immunoassay applications to detect and quantify TNF-α expression in biological samples.

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Lab products found in correlation

Fix perm, by BD (2 mentions) Anti ifn γ fitc, by BD (2 mentions) Golgistop, by BD (2 mentions) Brefeldin a, by BD (2 mentions) Anti mip1β pe, by BD (2 mentions) Anti cd107a pe cy5, by BD (2 mentions) Anti il 4 pe, by BD (1 mentions) Anti il 17a pe, by BD (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Human serum, by Merck Group (1 mentions) Interleukin 2 (il 2), by Novartis (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Anti il 5 apc, by BD (1 mentions) Cx3cr1 fitc, by BioLegend (1 mentions) Anti ifn γ v450 b27, by BD (1 mentions) Anti il22 apc, by R&D Systems (1 mentions)

3 protocols using anti tnf alexafluor700

1

Profiling immune cell responses in atopic dermatitis

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PBMC and skin samples were obtained from patients with AD from the Department of Dermatology or from healthy donors from the Department of Plastic Surgery. This study was approved by the Ethical Committee from the University Hospital of Lille. All subjects provided written informed consent. Patients with AD were selected according to the Hanifin and Rajka criteria (Hanifin and Rajka, 1980 ). PBMCs were obtained after elimination of granulocytes on a Ficoll gradient. Punch skin biopsies (diameter 5 mm) were cultured in complete RPMI 1640 supplemented with 100 U/ml penicillin, 100 µg/ml streptomycin (all Invitrogen), 5% human serum (Sigma-Aldrich), and 20 U IL-2/ml (Novartis). After 10–13 d, emigrating cells were collected and characterized by surface and intracellular staining by flow cytometry. Surface and intracellular cytokine staining was performed using the Cytofix/Cytoperm kit (BD) according to the manufacturer’s instructions. The following fluorochrome-conjugated antibodies were used: anti–IFN-γ–V450 (B27), anti-TNF–Alexa Fluor 700 (Mab11), anti–IL-4–PerCP-Cy5.5 (8D4-8), anti-IL17A-PE (N49-653), anti–IL-5–APC (TRFK5), anti–IL-9 PerCP-Cy5.5 (MH9A3), anti-CD4-APC-Cy7 (RPA-T4), anti–IL-13–Horizon-V450 (JES10-5A2; all BD), anti-IL4-PE (3010.211; BD), anti-IL22-APC (142928; R&D Systems), and CX3CR1-FITC (2A9-1; BioLegend).
Staumont-Sallé D., Fleury S., Lazzari A., Molendi-Coste O., Hornez N., Lavogiez C., Kanda A., Wartelle J., Fries A., Pennino D., Mionnet C., Prawitt J., Bouchaert E., Delaporte E., Glaichenhaus N., Staels B., Julia V, & Dombrowicz D. (2014). CX3CL1 (fractalkine) and its receptor CX3CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin. The Journal of Experimental Medicine, 211(6), 1185-1196.
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2

NK Cell Cytotoxicity Assay Protocol

Cited 1 time
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NK-cell responses to target cell lines were determined as previously described [18 (link)]. PBMC were incubated with culture media alone or with K562 cells, 221 cells or antibody-coated p815 cells at an effector to target ratio of 10:1 for 5 hours in the presence of anti-CD107a PE-Cy5 (BD Biosciences, 10 ml/mL), brefeldin A (2.5 mg/mL) and Golgistop (2.5 mg/mL, BD Bioscience). Cells were then washed, stained with the Fixable Blue Dead Cell Stain prior to staining for surface receptors, then fixed, permeabilized (BD Fix/perm, BD Biosciences) and stained with anti-IFNγ-FITC, anti-MIP1β-PE and anti-TNF-Alexafluor700 (all BD Biosciences). Gates were set on media-only controls, which were also used for background subtraction prior to analysis.
Yu W.H., Cosgrove C., Berger C.T., Cheney P.C., Krykbaeva M., Kim A.Y., Lewis-Ximenez L., Lauer G.M, & Alter G. (2018). ADCC-Mediated CD56DIM NK Cell Responses Are Associated with Early HBsAg Clearance in Acute HBV Infection. Pathogens & Immunity, 3(1), 2-18.
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3

NK Cell Cytotoxicity Assay Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
NK-cell responses to target cell lines were determined as previously described [18 (link)]. PBMC were incubated with culture media alone or with K562 cells, 221 cells or antibody-coated p815 cells at an effector to target ratio of 10:1 for 5 hours in the presence of anti-CD107a PE-Cy5 (BD Biosciences, 10 ml/mL), brefeldin A (2.5 mg/mL) and Golgistop (2.5 mg/mL, BD Bioscience). Cells were then washed, stained with the Fixable Blue Dead Cell Stain prior to staining for surface receptors, then fixed, permeabilized (BD Fix/perm, BD Biosciences) and stained with anti-IFNγ-FITC, anti-MIP1β-PE and anti-TNF-Alexafluor700 (all BD Biosciences). Gates were set on media-only controls, which were also used for background subtraction prior to analysis.
Yu W.H., Cosgrove C., Berger C.T., Cheney P.C., Krykbaeva M., Kim A.Y., Lewis-Ximenez L., Lauer G.M, & Alter G. (2018). ADCC-Mediated CD56DIM NK Cell Responses Are Associated with Early HBsAg Clearance in Acute HBV Infection. Pathogens & Immunity, 3(1), 2-18.
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