Mouse anti cdkn1a

Manufactured by Santa Cruz Biotechnology

Mouse anti-Cdkn1a is a primary antibody that specifically binds to the Cdkn1a protein in mouse samples. Cdkn1a is a cyclin-dependent kinase inhibitor that plays a role in cell cycle regulation.

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CDKN1A (8 citations) Anti-CDKN1A (2 citations)

2 protocols using mouse anti cdkn1a

Immunohistochemistry and in-situ Hybridization

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Tissues were fixed in 4% paraformaldehyde, embedded in paraffin wax, and sectioned at 5-μm intervals. Immunohistochemistry was performed using the following antibodies: mouse anti-phopho-histone3 (1:200; Cell Signaling Technology), goat anti-CC10 (1:20; Santa Cruz Biotechnology), rabbit anti-SP-C (1:500; Chemicon), rabbit anti-Nkx2.1 (1:50; Santa Cruz Biotechnology), mouse anti-Cdkn1a (1:100; Santa Cruz Biotechnology), rabbit anti-Sox9 (1:100; Santa Cruz Biotechnology), rabbit anti-Rbl2/p130 (1:50; Abcam), rabbit anti-Par3 (1:100; Upstate Biotechnology), rabbit anti-Sox2 (1:500; Seven Hills Bioreagents), and rabbit anti-Cdh1 (1:100; Cell Signaling). Slides were mounted with Vectashield mounting medium containing DAPI (Vector Laboratories). ISH was performed as described (Zhang et al. 2008 (link)). Probes were amplified from each lncRNA using the primers listed in Supplemental Table 8.

Herriges M.J., Swarr D.T., Morley M.P., Rathi K.S., Peng T., Stewart K.M, & Morrisey E.E. (2014). Long noncoding RNAs are spatially correlated with transcription factors and regulate lung development. Genes & Development, 28(12), 1363-1379.

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Immunoblot and Confocal Microscopy Analysis

Cited 2 times

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Immunoblot analysis was performed as previously described^{8 (link),35 (link)}, using rabbit anti-TFF3 polyclonal antibody. Mouse anti-β-actin, mouse anti-CDKN1A, mouse anti-BCL2, mouse anti-CDK2, rabbit anti-CDK4, rabbit anti-CCNE1, mouse anti-cSRC, and mouse anti-CCND1 antibody was obtained from Santa Cruz Biotechnology, CA. Rabbit anti-p-cSRC antibody were obtained from Cell Signaling, USA. Rabbit anti-pSTAT3(Tyr705) and mouse anti-STAT3 antibodies were obtained from Abcam, Cambridge, MA. Confocal microscopy scanning was performed as previously described^{51 (link)}. Secondary antibody, Alexa Fluor 488 goat anti-rabbit IgG was purchased from Invitrogen, Singapore. Rhodamine-conjugated phalloidin (Sigma, St Louis, MO) was used to visualize f-actin filaments.

Pandey V., Zhang M., You M., Zhang W., Chen R., Zhang W., Ma L., Wu Z.S., Zhu T., Xu X.Q, & Lobie P.E. (2018). Expression of two non-mutated genetic elements is sufficient to stimulate oncogenic transformation of human mammary epithelial cells. Cell Death & Disease, 9(12), 1147.

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