15n5 dg

Manufactured by Cambridge Isotopes

Sourced in United States

15N5-dG is a stable isotope-labeled nucleoside that contains five 15N atoms in place of the natural 14N atoms. This compound is used as a research tool in various applications, including mass spectrometry, nuclear magnetic resonance (NMR) spectroscopy, and tracer studies. The specific function of 15N5-dG is to serve as a labeled internal standard or tracer for analytical and biochemical investigations.

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Lab products found in correlation

Methanol, by Thermo Fisher Scientific (2 mentions) Aqueous ammonia, by Merck Group (1 mentions) Lc ms ultra chromasolv water, by Merck Group (1 mentions) Acetonitrile isocratic grade, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) 15n5 8 oxodg, by Cambridge Isotopes (1 mentions) Proteinase k, by Qiagen (1 mentions) Hplc grade water, by Thermo Fisher Scientific (1 mentions)

3 protocols using 15n5 dg

Quantification of 8-oxodG and 8-oxoGuo

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For this study, 8-oxo-7,8-dihydroguanosine (8-oxoGuo) was purchased from BioLog (Bremen, Germany) and 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) from Berry & Associates (Dexter, MI). The internal standards, ¹⁵N₅-8-oxodG and ¹⁵N₅-8-oxoGuo, were produced in the lab by electrochemical oxidation [16] (link) of ¹⁵N₅-dG and ¹⁵N₅-Guanosine, which were purchased from Cambridge Isotope Laboratories (Tewksbury, MA).
Acetonitrile (isocratic grade) was purchased from Merck KgaA (Darmstadt, Germany). Methanol (HPLC-grade), lithium acetate dihydrate, acetic acid, and aqueous ammonia (25%) were all from Sigma-Aldrich (Steinheim, Germany). LC-MS Ultra Chromasolv water from Sigma-Aldrich was used for preparation of the Lithium acetate buffer and mobile phase. QC samples were prepared from a pool of urine samples and stored at –20 °C.

Rasmussen S.T., Andersen J.T., Nielsen T.K., Cejvanovic V., Petersen K.M., Henriksen T., Weimann A., Lykkesfeldt J, & Poulsen H.E. (2016). Simvastatin and oxidative stress in humans: A randomized, double-blinded, placebo-controlled clinical trial. Redox Biology, 9, 32-38.

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Synthesis of Isotope-Labeled DNA Adducts

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[¹⁵N₅]-6-oxo-M₁dG was synthesized as described previously by substituting [¹⁵N₅]-dG (Cambridge Isotope Laboratories, Andover, MA, USA) for dG (15 (link)). Similarly, [¹³C, ¹⁵N₂]-M₁dG was synthesized as described previously by substituting [¹³C, ¹⁵N₂]-dG (Cambridge Isotope Laboratories, Andover, MA, USA) for dG (27 (link)).

Wauchope O.R., Mitchener M.M., Beavers W.N., Galligan J.J., Camarillo J.M., Sanders W.D., Kingsley P.J., Shim H.N., Blackwell T., Luong T., deCaestecker M., Fessel J.P, & Marnett L.J. (2018). Oxidative stress increases M1dG, a major peroxidation-derived DNA adduct, in mitochondrial DNA. Nucleic Acids Research, 46(7), 3458-3467.

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Analytical Standards for Oxidative DNA Adducts

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Nucleic acid purification grade lysis buffer, protein precipitation solution, and proteinase K were purchased from Gentra Systems (Minneapolis, MN). HPLC grade water and methanol were from Thermo Fisher Scientific Company (Raleigh, NC). ¹⁵N₅-8-oxo-dG, ¹⁵N₅-dG and ¹³C₁₀-dG were purchased from Cambridge Isotope Laboratories (Andover, MA, USA). Other chemical reagents were from Sigma-Aldrich Chemical Company (St. Louis, MO). ¹⁵N₅-1,N⁶-εdA standard was synthesized as described by Ham et al.^{62 (link)} 1,N²-εdG and ¹³C₁₀-1,N²-εdG were synthesized as reported by Kusmierek et al.^{63 (link)} MDA-modified ¹⁵N₅ and ¹⁴N₅ DNA were made by the method in Jeong's study.^{64 (link)} AcrdG, CrdG, and HNEdG standards and their ¹⁵N₅-labeled internal standards were synthesized according to previously published methods.^{65 (link)–67 (link)}

Mutlu E., Gao L., Collins L.B., Walker N.J., Hartwell H.J., Olson J.R., Sun W., Gold A., Ball L.M, & Swenberg J.A. (2016). Polychlorinated Biphenyls Induce Oxidative DNA Adducts in Female Sprague–Dawley Rats. Chemical research in toxicology, 29(8), 1335-1344.

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