Antibodies used were as follows: anti-Tyro3 (Santa Cruz, Dallas, TX, sc-1095, R&D Systems, Minneapolis, MN, AF759), anti-Axl (Santa Cruz sc-1097, R&D Systems AF854), anti-Mer (R&D Systems AF591), anti-HA (Covance, Princeton, NJ, MMS-101P, Roche, Mannheim, Germany 11 867 423 001), anti-Gas6 (R&D Systems AF986), anti-Akt (Cell Signaling, Beverly, MA, 4691), anti-Gapdh (Millipore, Billerica, MA, MAB374), anti-cleaved caspase 3 (Cell Signaling 9661), and anti-phosphotyrosine (Millipore 05–321). Human Pros1 was from Enzyme Research Laboratories, South Bend, IN, and Tet-Express from Clontech, Mountain View, CA.
Tet express
Manufactured by Takara Bio
The Tet-Express is a lab equipment product from Takara Bio. It is a technology platform that enables the regulation of gene expression through the use of tetracycline-responsive promoters. The core function of the Tet-Express is to provide researchers with a reliable and efficient tool for controlling and monitoring gene expression in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Human pros1, by Enzyme Research (3 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti tyro 3, by Santa Cruz Biotechnology (1 mentions)
Anti axl, by Santa Cruz Biotechnology (1 mentions)
Anti phosphotyrosine, by Merck Group (1 mentions)
Anti gas6, by R&D Systems (1 mentions)
Anti mer, by R&D Systems (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Anti ha, by Fortrea (1 mentions)
Polyjet, by SignaGen (1 mentions)
2 protocols using tet express
1
Tyrosine Kinase Receptor Signaling
2
Investigating APP Overexpression in Trophoblast Cells
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The functional implication of APP overexpression was investigated in a previously established trophoblast cell line HTR-8/SVneo. 17 HTR-8/SVneo was stably transfected with a Tet-On expression plasmid harboring APP ORF pTRE3G-BI-APP. Two clones, B2 and B10, were selected. APP was induced in B2 and B10 by including the recombinant protein Tet-Express (Clontech) in the culture medium.
For assessing the impact of APP overexpression on syncytialization, BeWo was first stably transfected with pCMV-TET3G, which expresses the Tet-On 3G transactivator protein. BeWo-tet3G was then transiently transfected with pTRE3G-BI-APP using PolyJet (Signagen, Rockville, MD), according to the manufacturer's protocol, and APP was induced with doxycycline.
For assessing the impact of APP overexpression on syncytialization, BeWo was first stably transfected with pCMV-TET3G, which expresses the Tet-On 3G transactivator protein. BeWo-tet3G was then transiently transfected with pTRE3G-BI-APP using PolyJet (Signagen, Rockville, MD), according to the manufacturer's protocol, and APP was induced with doxycycline.
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