Chitosan, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA), folic acid (FA), Doxorubicin (DOX), cisplatin (Cis.), N-Ethyl-N′ -(3-dimethylamino propyl) carbodiimide hydrochloride (EDAC), N-Hydroxysuccinimide (NHS), and sodium hydroxide pellets were purchased from Sigma Aldrich, St. Louis, MO, USA; ethanol was obtained from Baker Analyzed, Fisher Scientific, Landsmeer, The Netherlands. Apigenin (99.06% purity, CAS Number 520-36-5) was obtained from Chengdu Herbpurify Co., Ltd. (Wuhan, China). Hepatocellular carcinoma (HePG-2) and epithelioid carcinoma (Hela) cell lines were obtained from ATCC via Holding Company for Biological Products and Vaccines (VACSERA), Cairo, Egypt. Fetal Bovine serum (GIBCO, Carlsbad, UK), Annexin V-FITC Apoptosis Kit Plus BioVision. Qiagen RNA extraction/BioRad SYBR green PCR MMX. A catalase activity assay colorimetric kit (ab83464) and propidium iodide flow cytometry kit (ab139418) were purchased from Abcam. Superoxide dismutase (SOD) activity assay: Enzyme-linked Immunosorbent Assay Kit (Cloud-Clone Corp). All other reagents used were of analytical grade.
Annexin 5 fitc apoptosis kit plus
Manufactured by Abcam
Sourced in United States
The Annexin V-FITC Apoptosis Kit Plus is a laboratory product designed to detect and quantify apoptosis in cells. It contains Annexin V conjugated to the fluorescent dye FITC, which binds to phosphatidylserine exposed on the cell surface during apoptosis. The kit also includes a propidium iodide solution to identify late apoptotic or necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Ab83464, by Abcam (1 mentions)
Ab139418, by Abcam (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Enzyme linked immunosorbent assay kit, by Cloud-Clone (1 mentions)
N ethyl n 3 dimethylaminopropyl carbodiimide hydrochloride, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Folic acid, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Apigenin, by Chengdu Herbpurify (1 mentions)
Hepes buffer solution, by Procell (1 mentions)
Ezcell cell cycle analysis kit, by Abcam (1 mentions)
Cytoflex v0 b3 r1 flow cytometer, by Beckman Coulter (1 mentions)
5 protocols using annexin 5 fitc apoptosis kit plus
1
Multifunctional Chitosan-Based Anticancer Nanomedicine
2
Annexin V-FITC Apoptosis Assay
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Apoptosis/necrosis was examined using Annexin V-FITC Apoptosis Kit Plus (BioVision). The excitation/emission was detected at 488/530 nm wavelength.
3
SLAMF8-KD ALCL Cell Apoptosis
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We analyzed the apoptosis of ALCL cell lines using Annexin V-FITC Apoptosis Kit Plus (Biovision, Milpitas, CA, USA). Control and SLAMF8-KD ALCL cells were cultured for 24 h at 1–3 × 104 cells/100 µL/well in RPMI1640 + 10% FCS. The cells were analyzed according to the manufacturer’s instructions. The FITC-positive / PI-negative cells were considered apoptotic cells. Three individual experiments were performed.
4
Apoptosis Assay Using Annexin V-FITC
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Following 48 hours of transfection, cells were digested with EDTA-free 0.25% trypsin (YB15050057; YBio, Shanghai, China), collected in flow tubes, and centrifuged, and the supernatants were removed. Following three washes with cold PBS, the cells were centrifuged, and the supernatants were removed. This assay was conducted following the specifications of the Annexin V-FITC Apoptosis Kit Plus (K201-100; BioVision, Milpitas, CA, USA). The cells were resuspended in the staining solution, shaken, mixed, incubated at room temperature for 15 minutes, and shaken and mixed with 1 mL HEPES buffer solution (PB180325; Procell). Apoptosis was evaluated by flow cytometry.
5
Cell Cycle and Apoptosis Analysis
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Each cell line was treated with IC 50 of sterol fraction for 24 h in the cell culture flasks,. Cells were harvested, washed and counted to have not less than 3*105 cells/ each group with at least 90% living cells. Then the cells were stained with EZCellTM Cell Cycle Analysis Kit, BioVision, Inc., San Francisco Bay Area Catalog #K920-100. Another portion of cells was treated with Annexin V-FITC Apoptosis Kit Plus, BioVision Inc., San Francisco Bay Area. Apoptotic divisions were measured according to the Biovision protocol (Biovision.com. Annexin V-FITC Apoptosis Detection). CytoFLEX V0-B3-R1 Flow Cytometer, Beckman Coulter Life Sciences (Indianapolis, United States) was used.
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