The plasma AA concentration was analyzed with norleucine as an internal standard in an Automatic AA Analyzer (Hitachi High-technologies Corporation, Tokyo, Japan). To measure the plasma glucose levels, plasma was pretreated with ice-cold sulfosalicylic acid (50 g/L) at a ratio of 1:4 (v/v) to precipitate protein24 (link), followed by centrifugation at 8,320 × g for 30 min at 4 °C. The supernatant was filtered through 0.45-μm and 0.22-μm nylon syringe filter units (Fisher Scientific, Pittsburgh, PA) and placed in microcentrifuge tubes (catalog no. 05-664-34, Fisher Scientific). The plasma glucose concentrations were subsequently measured using an Auto Analyzer 7020 instrument (Hitachi High-technologies) and commercial colorimetric kit (DiaSys Diagnostics Systems GmbH, Frankfurt, Germany)25 (link).
Auto analyzer 7020 instrument
Manufactured by Hitachi
Sourced in Japan
The Hitachi Auto Analyzer 7020 is an analytical instrument designed for automated biochemical analysis. It is capable of performing a wide range of clinical chemistry tests, including tests for enzymes, proteins, and metabolites, among others. The instrument utilizes various analytical techniques, such as colorimetry and ion-selective electrodes, to provide accurate and reliable results.
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Lab products found in correlation
2 protocols using auto analyzer 7020 instrument
1
Plasma Amino Acid and Glucose Quantification
2
Calf Metabolic Profiles Before Colostrum
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Fourteen and fteen blood samples from each newborn calf was collected from the jugular vein before colostrum consumption in the CON and NCG group respectively. Then these blood samples centrifuged at 3,000 × g for 15 min at 4°C for collection of plasma and then frozen at -80°C until subsequent analysis. A subsample of each plasma sample was used to analyze biochemical variables, including glucose, total protein, blood urea nitrogen, non-esteri ed fatty acids, β-hydroxybutyrate, cholesterol, triglyceride, total bilirubin, albumin, and globulin using an AutoAnalyzer 7020 instrument (Hitachi High-technologies Corporation, Tokyo, Japan) with colorimetric commercial kits (Ningbo Medical System Biotechnology Co., Ltd.). The plasma AA compositions were determined as described elsewhere [19] . Six plasma subsamples in each group were collected based on the following criteria: parities of the dairy cows, number of days from the data, the cows began receiving NCG to the calving day, and calf sex (female) to analyze the metabolome pro les with GC-MS.
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