Adenosine diphosphate (adp)

Manufactured by Sangon

Sourced in China

ADP is a laboratory equipment product that facilitates the measurement of adenosine diphosphate (ADP) levels. It functions as a tool for biochemical analysis and research applications.

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Lab products found in correlation

Adenosine triphosphate (atp), by Sangon (3 mentions) Bsm dna polymerase, by Thermo Fisher Scientific (1 mentions) 4 aminomethyl trioxsalen hydrochloride amt, by Merck Group (1 mentions) Tris hydroxymethyl aminomethane, by Serva Electrophoresis (1 mentions) Methoxyamine hydrochloride, by Merck Group (1 mentions) D mannose 6 phosphate, by Merck Group (1 mentions) N methyl n trimethylsilyl trifluoroacetamide mstfa, by Merck Group (1 mentions) D glucose 6 phosphate, by Merck Group (1 mentions) Pyridine, by Merck Group (1 mentions) D glucose, by Sangon (1 mentions) Mgcl2, by Sangon (1 mentions)

4 protocols using adenosine diphosphate (adp)

Graphene Oxide-Assisted Enzymatic Assay

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Nb.bpu10I endonuclease and Bsm DNA polymerase were obtained from Thermo Scientific (Waltham, MA, USA). Graphene oxide (GO) was obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Deoxyribonucleoside triphosphate (dNTP), ATP, ADP, AMP, guanosine triphosphate (GTP), uridine triphosphate (UTP) and cytidine triphosphate (CTP) were all purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Human serum was purchased from XinFan Bio-technology Co., Ltd. (Beijing, China). In all experimental procedures, ultrapure water (18.2 MΩ cm) acquired from a Micropore filtration system was employed to prepare all solutions.
In this work, all the DNA sequences were synthesized and modified by Sangon Biotech Co., Ltd. (Shanghai, China). In the Supplementary Information, these sequences are presented in Table S1.

Chen X., Feng Y., Chen H., Zhang Y., Wang X, & Zhou N. (2022). Fluorescent Aptasensor for Highly Specific Detection of ATP Using a Newly Screened Aptamer. Sensors (Basel, Switzerland), 22(7), 2425.

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Photosensitizer-Based DNA Damage Protocol

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4′-Aminomethyltrioxsalen hydrochloride (AMT) purchased from Sigma (China, Shanghai); Tris(hydroxymethyl)aminomethane purchased from Serva (China, Shanghai); ATP (adenosine-5′-triphosphate disodium trihydrate); AMP (adenosine-5′-monophosphate disodium); CTP (cytidine-5′triphosphate), disodium salt GTP (GTP, guanosine-5′triphosphate); ADP (adenosine-5′-dlphosphate disodium salt), all purchased from Sangon Biotech Co., Ltd. (Shanghai). The solution was prepared from ultrapure water (18.2 MΩ•cm). The pH was measured using a lightning magnetic (Shanghai) pHS-3C acidity meter. DNA, purified by HPLC, was purchased from Sangon Biotech Co., Ltd. (Shanghai), and the DNA stock solution was prepared by ultrapure water. The base sequences of DNA used in this work are given in Table S1 (Supporting Information). Blood samples from healthy volunteers were supplied by Shangqiu First People's Hospital.

Wang D., Geng F., Wang Y., Ma Y., Li G., Qu P., Shao C, & Xu M. (2020). Design of a Fluorescence Turn-on and Label-free Aptasensor Using the Intrinsic Quenching Power of G-Quadruplex to AMT. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(8).

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Fabrication of ATP-Responsive Biosensor

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Melamine and Co(Ac)₂·4H₂O were purchased from Damao Chemical Reagent Factory (Tianjin, China). Cyanuric acid, 2-methylimidazole, Potassium chloride (KCl) yeast extract powder, peptone, and sodium chloride (NaCl) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO) was ordered from Tianjin Fuyu Fine Chemical Co., Ltd. (Tianjin, China). Methanol anhydrous was bought from Tianjin jindongtianzheng Precision Chemical Reagent Factory (Tianjin, China). K₃[Fe(CN)₆] and K₄[Fe(CN)₆]·H₂O were obtained from Tianjin Ruijinte Chemical Co., Ltd. (Tianjin, China). ATP, ADP, AMP, guanosine triphosphate (GTP), cytidine triphosphate (CTP), uridine triphosphate (UTP), the sequence of ATP-targeted aptamer and mutant sequence (Table S1, Supplementary Materials) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). The Escherichia coli BL21 (E. coli BL21; CICC 24719) was purchased from the Agricultural Culture Collection of China (Beijing, China). All reagents were used without any further purification.

Wang P., Zhang R., Wu Y., Chang Y, & Liu M. (2022). An Electrochemical Aptasensor Integrating Zeolitic Imidazolate Framework for Highly Selective Detection of Bioaerosols. Biosensors, 12(9), 725.

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Metabolite Extraction and Derivatization

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D-Erythrose 4-phosphate (E4P), glycolaldehyde, D-glucose 6-phosphate, D-mannose 6-phosphate, methoxyamine hydrochloride, pyridine and N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) were purchased from Sigma-Aldrich (St. Louis, MO, United States). D-Allose and D-altrose were purchased from Macklin Biochemical (Shanghai, China). Other regents such as D-glucose, D-mannose, ATP, ADP, thiamine pyrophosphate (TPP), and MgCl₂ were all purchased from Sangon Biotech (Shanghai, China), unless noted otherwise. Escherichia coli DH5α was used for plasmid construction and preservation. Escherichia coli BL21 (DE3) was used for protein expression. Luria-Bertani (LB) medium (10 g/L tryptone, 5 g/L yeast extract and 10 g/L NaCl) was used for E. coli cell culture and recombinant protein expression, supplemented with 50 μg/ml kanamycin or 100 μg/ml ampicillin when necessary.

Mao Y., Yuan Q., Yang X., Liu P., Cheng Y., Luo J., Liu H., Yao Y., Sun H., Cai T, & Ma H. (2021). Non-natural Aldol Reactions Enable the Design and Construction of Novel One-Carbon Assimilation Pathways in vitro. Frontiers in Microbiology, 12, 677596.

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