Goat anti mouse cy3

All tissue culture reagents were from Euroclone SpA except ECGS and heparin (Sigma Aldrich, cat #E2759 and #H3149, respectively). N^G-Nitro-L-arginine methyl ester (L-NAME, cat #N5751), methylcellulose (cat #M0512), FITC-labelled phalloidin (cat #P5282), and DAPI (cat #D9542) were from Sigma Aldrich; collagenase (cat #17454) and rat tail collagen I (cat #47254) from Serva; recombinant human VEGF₁₆₅ (cat #100) from Peprotech; mitomycin (cat #BIA-M1183) from Tebu-bio. Primary antibodies used were: mouse monoclonals anti-eNOS (BD Transduction Laboratories, cat #610296) and anti-β-actin (Sigma Chemicals, cat #A2228), and rabbit polyclonal anti phospho-eNOS (Ser1177) (Cell Signalling Technology, cat #9571). HRP-conjugated secondary antibodies were from Dako (cat #P0260 and #P0399 for rabbit anti-mouse and swine anti-rabbit antibodies, respectively). A goat anti-mouse CY3 (cat #29-0382-75, GE Healthcare) was used in immunofluorescence experiments to detect eNOS.

The anti-CD31 antibody (DIA310; 1:300) was purchased from Dianova (Hamburg, Germany). The anti-α-smooth muscle actin (SMA) (Ab5694; 1:500), anti-insulin (Ab7842; 1:300), anti-somatostatin (Ab30788; 1:300) and anti-glucagon (Ab92587; 1:300) antibodies were purchased from Abcam (Cambridge, UK). The goat-anti-rabbit-cyanine (Cy)3 (1:1000); goat-anti-mouse-Cy3 (1:1000); goat-anti-rat-Cy3 (1:1000); goat-anti-rat-AlexaFluor555 (1:1000) and goat-anti-rabbit-streptavidin-peroxidase (1:500) antibodies were purchased from GE Healthcare (Freiburg, Germany).