After incubation with SePTX NPs for 48 h, the cells were incubated in 4% paraformaldehyde for 20 min, 1% of bovine serum albumin was used for cell blocking, p53 (ab78316; Abcam) or LC3-II (ab48394; Abcam) was used for incubation of primary antibodies. The blocking time was 30 min at room temperature and the primary antibody was incubated overnight at 4oC. Cy3-labeled goat anti-rabbit IgG (A0516; Beyotime) was used for incubation of the secondary antibody, and the secondary antibody were incubated for 1.5 h at 4oC. DAPI was used for staining of nuclei, and confocal microscopy (Carl Zeiss LSM780; Instrument Development Center, NCKU, Tainan City, Taiwan) was used for observation of intracellular fluorescence intensity.
Cy3 labeled goat anti rabbit igg a0516
Manufactured by Beyotime
Cy3-labeled goat anti-rabbit IgG (A0516) is a secondary antibody used for detection and visualization in various immunoassays and immunohistochemical techniques. It is conjugated with the Cy3 fluorescent dye, which has an excitation maximum at 550 nm and an emission maximum at 570 nm.
Automatically generated - may contain errors
2 protocols using cy3 labeled goat anti rabbit igg a0516
1
Immunofluorescence Analysis of p53 and LC3-II
2
Immunofluorescence Analysis of FSHR and AMH
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HGCs were
cultured on coverslips (14 mm, NEST) at 5 × 104 cells/mL
in 6-well plates for 2 days. Then, the coverslips were washed twice
with PBS and fixed in 4% paraformaldehyde (PFA). After protein blocking
(C0265, Beyotime, China) for 30 min at 37 °C, the coverslips
were incubated with an antibody against follicle-stimulating hormone
receptor (FSHR, 1:100, 22665-1-AP, Proteintech, China) and an antibody
against anti-Müllerian hormone (AMH, 1:100, 23479-1-AP, Proteintech)
diluted in PBS overnight at 4 °C. The next day, all the coverslips
were washed and incubated with secondary antibodies (1:500, Cy3-labeled
goat antirabbit IgG, A0516, Beyotime) at room temperature for 2 h.
4′,6′-Diamidino-2-phenylindole (DAPI, C1005, Beyotime)
was used to visualize nuclei. Images were observed and captured using
an Olympus IX73 inverted microscope (Olympus, China).
cultured on coverslips (14 mm, NEST) at 5 × 104 cells/mL
in 6-well plates for 2 days. Then, the coverslips were washed twice
with PBS and fixed in 4% paraformaldehyde (PFA). After protein blocking
(C0265, Beyotime, China) for 30 min at 37 °C, the coverslips
were incubated with an antibody against follicle-stimulating hormone
receptor (FSHR, 1:100, 22665-1-AP, Proteintech, China) and an antibody
against anti-Müllerian hormone (AMH, 1:100, 23479-1-AP, Proteintech)
diluted in PBS overnight at 4 °C. The next day, all the coverslips
were washed and incubated with secondary antibodies (1:500, Cy3-labeled
goat antirabbit IgG, A0516, Beyotime) at room temperature for 2 h.
4′,6′-Diamidino-2-phenylindole (DAPI, C1005, Beyotime)
was used to visualize nuclei. Images were observed and captured using
an Olympus IX73 inverted microscope (Olympus, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!