Amersham phastsystem

Manufactured by GE Healthcare

The Amersham™ PhastSystem is a compact and versatile lab equipment designed for electrophoresis applications. It provides a controlled environment for the separation and analysis of biomolecules, such as proteins and nucleic acids. The system includes various components that work together to facilitate efficient and reliable electrophoresis experiments.

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Lab products found in correlation

Acrylamide gradient gels, by GE Healthcare (1 mentions) Endosafe pts, by Charles River Laboratories (1 mentions)

2 protocols using amersham phastsystem

CARD9-BCL10 Interaction Characterization

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Formation of the complex between CARD9 and BCL10 CARDs was assessed using native (non-denaturing) PAGE on an Amersham^™ PhastSystem (GE Healthcare Life Sciences) with pre-made 8–25% acrylamide gradient gels (GE Healthcare Life Sciences). Coomassie Brilliant Blue dye was used for the detection of protein band patterns. Separately purified CARD9 and BCL10 CARDs were pre-incubated together for 1 h at room temperature and the mixture was applied to the gel. Complex assembly was evaluated based on the appearance of newly formed bands and disappearance of existing bands.

Park J.H., Choi J.Y., Mustafa M.F, & Park H.H. (2017). In vitro reconstitution of interactions in the CARD9 signalosome. Molecular Medicine Reports, 16(4), 3910-3916.

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Antibody Purity and Endotoxin Analysis

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Purified recombinant antibodies were assessed by SDS-PAGE under non-reducing conditions using the Amersham Phastsystem™ (GE Healthcare) according to the manufacturer’s instructions. Endotoxin levels were measured on Endosafe®-PTS™ (Charles River) according to the manufacturer’s instructions.

Imbrechts M., Maes W., Ampofo L., Van den Berghe N., Calcoen B., Van Looveren D., Kerstens W., Rasulova M., Vercruysse T., Noppen S., Abdelnabi R., Foo C., Hollevoet K., Maes P., Zhang X., Jochmans D., Ven K., Lammertyn J., Vanhoorelbeke K., Callewaert N., De Munter P., Schols D., Thibaut H.J., Neyts J., Declerck P, & Geukens N. (2022). Potent neutralizing anti-SARS-CoV-2 human antibodies cure infection with SARS-CoV-2 variants in hamster model. iScience, 25(8), 104705.

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