Fecal dna rapid extraction kit

P. hominis was identified by PCR analysis (Supplemental Material; Figures S1, S2). Also, 25 fecal samples were examined by nested PCR for detection of Giardia duodenum and Cryptosporidium parvum (Supplemental Material; Figures S3–S6). DNA was extracted from each of the fecal samples using a Fecal DNA Rapid Extraction Kit (TIANGEN, Beijing, China), according to the manufacturer’s instructions (Zhang et al., 2019a (link)).

DNA was extracted from each fecal specimen using a Fecal DNA Rapid Extraction Kit (TIANGEN, Beijing, China) following the manufacturer’s instructions. All DNA samples were detected for the presence of P. hominis by nested PCR amplifying the partial 18S rRNA gene as previously described [27 (link)]. In addition, we performed TA cloning for each positive sample and amplified the ITS1/5.8S rRNA gene/ITS2 genomic region as previously described [35 (link)]. The genomic DNA extracted from P. hominis (ATCC 3000) and ddH₂O were used as positive and negative controls, respectively. All DNA specimens were analyzed twice. The positive PCR products were purified and sequenced.

The fresh fecal samples from 336 individuals were collected in empty containers and were cooled with ice during transport to the lab. All of the samples were stored at -20 °C until DNA extraction. In order to avoid the risk of cross-contamination, standard procedures for cleaning workspaces and instruments were applied. In addition, internal reference controls were set up at the same time.
Genomic DNA was extracted from each fecal sample using the Fecal DNA Rapid Extraction Kit (TIANGEN, Beijing, China) according to the manufacturer's instructions. The purified DNA samples were stored at -20 °C until PCR analysis.