Aquilion one ct scanner

The CT recordings were acquired using a Toshiba Aquilion ONE CT-Scanner at the radiology department of the Uniklinik Freiburg. To enhance the contrast between blood and tissue, contrast agent (Imeron 300M by Bracco) was injected with a dosage of approximately 2 mL/kg (based on guidelines for infants) at a flow of 0.2 mL/s (lowest possible rate). The resulting image resolution was 0.351 mm * 0.351 mm * 0.5 mm, with an acquisition window of 18 cm * 18 cm * 32 cm. During the recording, the rabbit (wildtype New Zealand white, female, 6 months, 3.0 kg) was anesthestized with S-ketamine and xylazine (12.5/3.75 mg/kg intramuscularly (IM), followed by 1–2.5 mL/kg/h intravenously (IV) to maintain anesthesia during the duration of CT). This anesthesia protocol was chosen as it does not appear to have a significant impact on cardiac repolarization, see Odening et al. [15 (link)]. All animal care and experimental procedures were performed in compliance with EU legislation (directive 2010/63/EU) and the German (TierSchG and TierSchVersV) animal welfare law, after approval by the local Institutional Animal Care and Use Committees in Germany (Regierungspraesidium Freiburg; approval number G18/118). Animal housing and handling was in accordance with good animal practice as defined by the Federation of European Laboratory Animal Science Association, FELASA.

Images were acquired using a Toshiba Aquilion ONE CT scanner (Toshiba Medical Systems Corporation,Otawara, Japan) using a standard acquisition protocol. Details of the protocol used for the head CT procedures are presented in Table 2.
Measurements of the skull were performed on workstation software (Version 4.82, ER001) on transaxial image of the skull. The biparietal diameter (BPD, width), defined as the distance between most projecting points at either side of the skull, which is usually just superior and posterior to the external auditory meatus (EAM), and the occipitofrontal diameter (OFD, length), defined as the distance from the glabella to the most projecting point at the back of the skull. Both measurements were made using a digital calliper within the workstation software to calculate the CI, which is expressed as: CI =BPD/OFD × 100.