Gm21756

Manufactured by Coriell Institute for Medical Research

Sourced in United States

GM21756 is a laboratory equipment product. It is used for a core function in scientific research, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Gm04693, by Coriell Institute for Medical Research (1 mentions) Freestyle 293 expression medium, by Thermo Fisher Scientific (1 mentions) Gm02153, by Coriell Institute for Medical Research (1 mentions)

3 protocols using gm21756

Huntington's Disease Fibroblast Culture

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Huntington’s disease-afflicted patient fibroblasts (GM21756)were purchased from the Coriell Institute. They were cultured in Dermal Fibroblast Growth Media (DFGM) supplemented with 10% (v/v) FBS and 1% Penicillin/Streptomycin. Fibroblasts were plated as 5×10³ cells/well on 96-well plates for transduction experiments.

Schmitt-Ulms C., Kayabolen A., Manero-Carranza M., Zhou N., Donnelly K., Nuccio S.P., Kato K., Nishimasu H., Gootenberg J.S, & Abudayyeh O.O. (2024). Programmable RNA writing with trans-splicing. bioRxiv.

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Huntington's Disease Cell Lines Protocol

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Immortalized HdhQ111 mutant striatal cell lines and HdhQ7 wt cells were obtained from the CHDI Foundation. Cells were cultured in DMEM supplemented with 10% FBS, 100 mg/ml penicillin, 100 mg/ml streptomycin, and 400 mg/ml G418. Cells were grown at 33°C in a 5% CO2 incubator. Cells within 14 passages were used in all experiments.
HD patient fibroblasts (HD1: GM04693, HD2: GM21756, purchased from Coriell Institute, USA) and normal fibroblasts (Con 1, fibroblasts from adult, HDFa; Con 2, fibroblasts from juvenile, HDFn; purchased from Sigma-Aldrich) were maintained in MEM supplemented with 15% (vol/vol) FBS and 1% (vol/vol) penicillin/streptomycin. All of the above cells were maintained at 37°C in 5% CO2–95% air.

Fu Z., Liu F., Liu C., Jin B., Jiang Y., tang M., Qi X, & Guo X. (2019). Mutant Huntingtin inhibits the mitochondrial unfolded protein response by impairing ABCB10 mRNA stability. Biochimica et biophysica acta. Molecular basis of disease, 1865(6), 1428-1435.

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Culturing Human Cell Lines

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Human fibroblast cell lines GM04204, GM04210, GM04230, GM04212, GM04208, GM04220, GM21756, GM09197, and GM02153 were purchased from the Coriell Institute for Medical Research and cultured in minimum essential medium with 15% fetal bovine serum (FBS). HeLa S3 cells were cultured in RPMI 1640 with 10% FBS. Human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium supplied with 10% FBS, and HEK 293GnTI⁻ cells were grown in suspension in FreeStyle 293 Expression Medium (Gibco) supplemented with 1% FBS. All human cell lines were cultivated at 37 °C in a humidified atmosphere containing 5% CO₂.

Chan K.Y., Li X., Ortega J., Gu L, & Li G.M. (2021). DNA polymerase θ promotes CAG•CTG repeat expansions in Huntington’s disease via insertion sequences of its catalytic domain. The Journal of Biological Chemistry, 297(4), 101144.

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