Anti-FLAG mouse monoclonal antibody (H3663) was purchased from Sigma-Aldrich (St. Louis, MO). Mouse anti-Myc (M200212F), Anti-FLAG agarose (M20018), anti-β-actin (M20009F) monoclonal antibody and rabbit anti-PRMT5 (T55454) were purchased from Abmart (Shanghai, China). Horseradish peroxidase (HRP ) labeled anti-mouse and anti-rabbit IgG antibodies were purchased from KPL (Milford, MA). Cell lysis buffer NP-40 (P0013F, 50 mM Tris-HCl [pH7.4], 150 mM NaCl, 1% NP-40) was used for Western blotting. Immunofluorescence secondary antibodies including fluorescein isothiocyanate (FITC )-labeled goat anti-mouse antibody (A0562), the dual luciferase reporter detection kit (RG027) and puromycin (ST551) were purchased from Beyotime (Shanghai, China). Exfect Transfection Reagent (T101-01/02) and T7 Endonuclease I Assay (T7E1) (EN303-01) were both purchased from Vazyme Biotechnology (Nanjing, China). SynScript III cDNA synthesis Mix (TSK322S) and 2 × TSINGKE Master qPCR Mix (TSE201) were purchased from TSINGKE Biotechnology (Beijing, China). The enhance chemiluminescence (ECL ) reagent (34075) was purchased from Thermo Fisher (Waltham, MA).
Exfect transfection reagent t101 01 02
Manufactured by Vazyme
Sourced in China
Exfect Transfection Reagent (T101-01/02) is a lipid-based transfection reagent developed by Vazyme for efficient and gentle delivery of nucleic acids, such as plasmid DNA, into a variety of mammalian cell lines. It facilitates the uptake of these molecules into cells, allowing for their subsequent expression or study.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using exfect transfection reagent t101 01 02
1
Reagents and Antibodies for Cell Biology Research
2
Antibody Reagents for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse anti-β-actin (M1210-2), anti-histone H3 (R1105-1), anti-glutathione S-transferase (GST; M0807-1) mAbs, and rabbit anti-GFP (SR48-02), anti-β-tubulin (0807-2), anti-FLAG (0912-1), and anti-MYC (R1208-1) pAbs were obtained from Huaan Biological Technology (Hangzhou, China). Rabbit mAb against DDX21 (ab182156) was purchased from Abcam (Cambridge, MA). Mouse mAbs to Cap and Rep of PCV2 were produced by our laboratory (Liu et al., 2005 (link)). Anti-FLAG affinity resin (A2220) was obtained from Sigma–Aldrich. The ExFect transfection reagent (T101-01/02) was obtained from Vazyme Biotechnology (Nanjing, China). All the antibodies and reagents were purchased and described previously (Zhou et al., 2020 (link), 2022a (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!