Plaque reduction neutralization tests were done as described before (Wölfel et al., 2020 (link)). In short, VeroE6 cells (4 × 105 cell/mL) were seeded in 24-well plates the day before. Prior to PRNT patient plasma were heat-inactivated at 56 °C for 30 min and diluted 1:20 up to 1:640. For each dilution step PRNT testing was done in duplicates. For PRNT samples were diluted in OptiPro (Fisher scientific, Schwerte, Germany) and mixed 1:1 with virus solution containing 100 plaque forming units of SARS-CoV-2 (EPI ISL 406862) and incubated at 37 °C for 1 h. The solution was added onto two wells of a 24-well plate. After 1 h at 37 °C the supernatants were discarded, the cells were washed once with PBS and supplemented with 1.2 % Avicel solution in DMEM (Merck, Darmstadt, Germany). After 3 days at 37 °C, the supernatants were removed and the 24-well plates were fixed and inactivated using a 6 % formaldehyde/PBS solution and stained with crystal violet as described (Herzog et al., 2008 (link)).
Avicel solution
Manufactured by Merck Group
Sourced in Germany
Avicel solution is a laboratory product manufactured by Merck Group. It is a microcrystalline cellulose suspension used as a component in various pharmaceutical and analytical applications. The core function of Avicel solution is to provide a stable and dispersed medium for various substances in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using avicel solution
1
SARS-CoV-2 Plaque Reduction Neutralization Test
2
Immobilized Cellulase from Spent Coffee Grounds
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Spent coffee grounds, used for the cellulase immobilization, are the waste material, discarded after espresso coffee beverage preparation (using Italian Frog Espresso Coffee Pod Machine, power: 450W, voltage: 230/120 V, under the following conditions: 7 g of ground coffee were extracted under the pressure within 25 s at about 90 °C) in the local cafe. The coffee was produced from “Doncafé - Espresso Aromatico - cialde”, Strauss Adriatic d.o.o., Šimanovci, Belgrade, Serbia and contained a mix of about 40% of Arabica, originated from Santos, Guatemala, Columbia, India and Honduras and around 60% of Robusta originated from India and Vietnam (depending on the current offer on the market, while taking into account similarity in composition). The working 1% Avicel solution (w/v), purchased from Merck, Germany, has been prepared fresh in tri-sodium citrate buffer (Na3C6H5O7), pH 4.8. The chlorine dioxide (ClO2) solution has been prepared by using 27% (w/v) sodium chlorite (NaClO2) and 10% (w/v) citric acid (C6H8O7), purchased from ACRŌS ORGANIC, New Jersey, USA and Lachema, Czech Republic, respectively, in ratio NaClO2: C6H8O7: dH2O = 1:5:14 (30% ClO2 solution). The potassium chloride (KCl) and magnesium chloride (MgCl2) salts were procured by Lachema, Czech Republic.
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