Carbendazim

Manufactured by LGC

Sourced in Germany

Carbendazim is a broad-spectrum fungicide used in various laboratory applications. It functions by inhibiting microtubule assembly, which disrupts cell division and growth in fungi. Carbendazim is commonly used in research and analytical settings to control fungal contamination in samples, cultures, and other laboratory materials.

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Lab products found in correlation

Tebuconazole, by LGC (4 mentions) Chlorpyrifos, by LGC (3 mentions) Imidacloprid, by LGC (3 mentions) Acetamiprid, by LGC (3 mentions) Difenoconazole, by LGC (3 mentions) Prochloraz, by LGC (2 mentions) Fenpropathrin, by LGC (2 mentions) Thiophanate methyl, by LGC (2 mentions) Methanol, by Merck Group (2 mentions) Triadimefon, by LGC (2 mentions) Pyrimethanil, by LGC (1 mentions) Milli q ultrapure water system, by Merck Group (1 mentions) Thiamethoxam, by LGC (1 mentions) Anhydrous magnesium sulfate mgso4, by Sinopharm (1 mentions) Acetic acid, by Sinopharm (1 mentions) Methanol, by Tedia (1 mentions) Formic acid, by Sinopharm (1 mentions) Chlorantraniliprole, by LGC (1 mentions) Azoxystrobin, by LGC (1 mentions) Pyraclostrobin, by LGC (1 mentions)

8 protocols using carbendazim

Pesticide Residue Analysis in Rape Flowers

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A Milli-Q ultrapure water system manufactured by Millipore (Milford, UT, USA) was used throughout experiments to provide the HPLC-grade water that was employed during analysis and to hydrate the rape flower samples. Ultragradient HPLC-grade acetonitrile and methanol were obtained from Tedia (Shanghai, China). Formic acid, acetic acid, anhydrous magnesium sulfate (MgSO₄), and sodium acetate (NaOAc) were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). PSA (primary and secondary amine-bonded silica), C₁₈ (octadecyl-bonded silica) and GCB (graphitized carbon black) were purchased from Agilent Technologies (Santa Clara, CA, USA).
Reference standards of imidacloprid (purity, 98%), thiamethoxam (purity, 95%), acetamiprid (purity, 95%), fenpropathrin (purity, 98.5%), chlorpyrifos (purity, 98%), carbendazim (purity, 95%), prochloraz (purity, 98%) and pyrimethanil (purity, 95%) were obtained from Dr. Ehrenstorfer GmbH (Augsburg, Germany).
Formulated pesticides of imidacloprid 10% wettable powder (WP), thiamethoxam 25% water-dispersible granules (WDG), acetamiprid 20% WP, fenpropathrin 20% emulsifiable concentrate (EC), chlorpyrifos 40% EC, carbendazim 80% WP, prochloraz 25% EC, and pyrimethanil 40% suspension concentrate (SC) were purchased from the agricultural market located in Hefei, Anhui, China.

Tong Z., Duan J., Wu Y., Liu Q., He Q., Shi Y., Yu L, & Cao H. (2018). Evaluation of Highly Detectable Pesticides Sprayed in Brassica napus L.: Degradation Behavior and Risk Assessment for Honeybees. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(10), 2482.

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Pesticide and Heavy Metal Analysis Protocol

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All pesticides standards including α-BHC(100 mg L⁻¹), β-BHC (100 mg L⁻¹), γ-BHC (100 mg L⁻¹), δ-BHC (100 mg L⁻¹), p,p′-DDE (100 mg L⁻¹), o,p′-DDT (100 mg L⁻¹), p,p′-DDT (100 mg L⁻¹), p,p′-DDD (100 mg L⁻¹), azoxystrobin (99.0%), carbendazim (99.0%), chlorothalonil (98.9%), chlorpyrifos (99.5%), cypermethrin (98.2%), chlorantraniliprole (98.2%), dichlorvos (99.7%), difenoconazole (99.2%), fenpropathrin (98.9%), isocarbophos (98.9%), procymidone (99.5%), prochloraz (99.2%), pyraclostrobin (98.1%), thiophanate-methyl (99.3%), tebuconazole (99.5%) and λ-cyhalothrin (93.5%) were purchased from Dr Ehrenstorfer GmbH (Augsburg, Germany). Acetonitrile (HPLC grade) were provided by Bangyi Chemical Co., Ltd. (Hangzhou, China). Anhydrous magnesium sulphate, anhydrous sodium sulphate and n-hexane were of analytical grade. Primary secondary amine (PSA) was provided by Agela Technologies.
Each single element stock solution (1000 mg L⁻¹) of Cu, Zn, Cr, Ni, Pb, As, Cd, and Hg were purchased from National Standard Material Research Centre (Beijing, China). Guaranteed reagent nitric acid (HNO₃) was provided by Fanhong Trading Co., Ltd. (Guangzhou, China).

Zhang Z., Song J., Zhang H., Zheng Z., Li T., Wu S., He B., Mao B., Yu Y, & Fang H. (2022). Analysis method development and health risk assessment of pesticide and heavy metal residues in Dendrobium Candidum. RSC Advances, 12(11), 6869-6875.

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Analytical Protocol for Carbendazim Quantification

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Methanol and acetonitrile with LC-MS grade were purchased from Merck (Darmstadt, Germany). Acetic acid (≥99.7% purity) was obtained from Sigma-Aldrich (St. Louis, USA). The Oasis HLB cartridges (6 cc/150 mg, 30 μm) were provided by Waters Ltd. (USA). QuEChERS Extraction kits (1.5 g anhydrous sodium acetate and 6 g anhydrous MgSO₄, 50 mL centrifuge tubes) and QuEChERS Clean-Up kits (150 mg anhydrous MgSO₄, 50 mg Primary and Secondary Amine (PSA), and 50 mg C₁₈, 2 mL centrifuge tubes) were purchased from Phenomenex Inc. (USA). Water (18.2 MΩ·Cm) used for reagent and sample preparation was from a Barnstead Nanopure system (Thermo Scientific, USA). All other chemicals were of analytical reagent grade. Analytical standards of carbendazim (purity 99.0%) and the internal standard carbendazim-D3 (purity 99.0%) were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). Stock standards (approximately 100 mg/L) of the individual compounds were prepared by dissolving the reference compounds in Methanol. Working standards at lower concentrations were prepared by serial dilution of the stock standards by blank matrixes.

Li Y.H., Zhou B.L., Qian M.R., Wang Q, & Zhang H. (2017). Transfer Assessment of Carbendazim Residues from Rape Flowers to Apicultural Products. Journal of Analytical Methods in Chemistry, 2017, 6075405.

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Analytical Standards for Pesticide Detection

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Analytical standards of flubendiamide (97%), triadimefon (99%), tebuconazole (99%), difenoconazole (98%), carbendazim (98%), fenaminosulf (99%), and thiophanate-methyl (99%) were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany), and analytical standards of triazophos, isoprothiolane, hexaconazole, propiconazole, prochloraz, tricyclazole, abamectin, buprofezin, emamectin benzoate, chlorantraniliprole, pymetrozine, imidacloprid, nitenpyram, thiamethoxam, acetamiprid, iprodione, procymidone, and diniconazole (all at 100 μg/ml) were bought from the Agro-Environmental Protection Institute (Tianjin, China). Methanol (MeOH; LC grade) and acetonitrile (ACN; LC grade) were obtained from Thermo Fisher Scientific, Inc. (Fairlawn, USA). Ammonium acetate (HPLC grade) and formic acid (HPLC grade) were provided by Tedia Company, Inc. (Fairfield, USA). Primary-secondary amine (PSA, 40–63 µm) and octadecyl silane (C₁₈, 50 µm) sorbents were purchased from Shanghai Anpel Scientific Instrument Co., Ltd. (Shanghai, China). Purified water was prepared by a Milli-Q reagent water system (Millipore, Milford, MA, USA).

Xu F., Yu J.Y., Wang Q.S., Fu Y., Zhang H, & Wu Y.L. (2019). Simultaneous determination of 25 pesticides in Zizania latifolia by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry. Scientific Reports, 9, 10031.

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Quantitative Analysis of Pesticide Residues in Fruits

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HCl (Carlo Erba, Cornaredo, Italy) was used to prepare the analyte (TBZ). All salt solutions for interference measurements, L(+)-ascorbic acid (GRAM-MOL, Zagreb, Croatia), citric acid (GRAM-MOL, Zagreb, Croatia), glucose (Kemika, Zagreb, Croatia), fructose (Kemika, Zagreb, Croatia), imazalil (Sigma Aldrich, St. Louis, MO, USA), fuberidazole (Dr. Ehrenstorfer, Augsburg, Germany), carbaryl (Dr. Ehrenstorfer, Augsburg, Germany), carbendazim (Dr. Ehrenstorfer, Augsburg, Germany), 2-phenylphenol (Sigma Aldrich, St. Louis, MO, USA) and NaOH (GRAM-MOL, Zagreb, Croatia) were prepared using analytical grade chemicals. Deionized water (conductivity of 0.055 µS/cm) was used to prepare all solutions.
Bananas, oranges, lemons, clementines, and limes were purchased from the local store in Osijek (Croatia) and were not declared bio fruit. Bananas originated from Ecuador, oranges and clementines from Greece, and lemons and limes from Spain.

Dandić A., Novak I., Jozanović M., Pukleš I., Széchenyi A., Budetić M, & Samardžić M. (2022). A New, MWCNT-Based, Solid-State Thiabendazole-Selective Sensor. Sensors (Basel, Switzerland), 22(10), 3785.

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Pesticide Residue Quantification Protocol

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Methanol, acetone, dichloromethane (DCM), ethyl acetate and acetonitrile were all purchased from Merck (Darmstadt, Germany) and Fisher Scientific (Trinidad, UK). All chemicals were of chromatographic reagent grade. Formic acid was obtained from Dikma Technologies (USA). Analytical-grade standards (purities>98%), carbendazim, imidacloprid, acetamiprid, triadimenol, triadimefon, tebuconazole, chlorbenzuron, difenoconazole, chlorpyrifos, buprofezin, and pyridaben were purchased from Dr. Ehrenstorfer (Augsburg, Germany) and Sigma-Aldrich (Steinheim, Germany). Individual chemical stock solutions (2 mg/mL) were prepared in HPLC-grade Methanol and stored in the dark at -20ºC. Anhydrous sodium sulfate (Na 2 SO 4 ), primary-secondary amine (PSA) bonded silica bulk, octadecylsilane (LC-C18, 40-63 μm) and nylon syringe filters (0.22 μm) were obtained from Shanghai Anpel Scientific Instrument Co., Ltd. (Shanghai, China). Ultra-pure water was obtained from a Milli-Q system (Bedford, MA, USA).

Shi R., Li Y., Chen M., Zheng X., Liu X., Zhao Y., Liu A., Jia J., Xu M., & Zhao Z. (2020). Detection of Frequently Used Pesticides in Apple Orchard Soil in China by High Resolution Mass Spectrometry. Polish Journal of Environmental Studies, 29(2), 1341-1350.

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Pesticide Extraction and Quantification

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All the organic solvents were of analytical grade. Acetone, acetonitrile, ethyl acetate, and toluene were purchased from J.T. Baker (Radner, Pennsylvania, USA). Sodium chloride, sodium sulfate, potassium dihydrogen phosphate, and dipotassium hydrogen phosphate were purchased from Merck (Darmstadt, Germany). The graphite carbon black (GCB) was purchased from Agilent Technologies (USA). The standard pesticides: 20 organophosphate (OP) pesticides, 10 carbamates, and 5 synthetic pyrthroids, and bifenthrin, carbendazim, macozeb, abamectin, and imidacloprid were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). Triphenylphosphate was purchased from Fluka (Buchs, Germany).

Prapamontol T., Hongsibsong S., Naksen W., Kerdnoi T., Kawichai S., Polyiem W., Pakvilai N., Phansawan B., & Santasup C. (2020). Multiple Pesticide Residues Found in Vegetables and Fruits from Rural and Urban Markets in Upper Northern Thailand. Chiang Mai University Journal of Natural Sciences, 20(1).

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Pesticide Residue Analysis Protocol

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Standard chlorpyrifos, beta-cypermethrin, tebuconazole, acetamiprid, and carbendazim materials (all purity > 97%) were obtained from Dr. Ehrenstorfer GmbH (Augsburg, Germany). Acetone, n-hexane, acetonitrile, anhydrous magnesium sulfate, and sodium chloride were used for pesticide residue analysis and were purchased from Beijing Chemical and Reagent (Beijing, China). Chromatography grade acetonitrile was obtained from Honeywell International Inc. (New Jersey, USA). Primary secondary amine and Florisil (1000 mg/6 mL) solid-phase extraction (SPE) columns were purchased from Agela Technologies (Tianjin, China). Water was purified (18.0 MU cm at 25 C) using an ultrapure purification system (Millipore, MA, USA).

Li M., Liu Y., Fan B., Lu J., He Y., Kong Z., Zhu Y., Jian Q, & Wang F. (2015). A chemometric processing-factor-based approach to the determination of the fates of five pesticides during apple processing. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 63(2).

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