Mle 12

Manufactured by BNCC

The MLE-12 is a laboratory equipment product. It is designed for specific core functions within a laboratory setting. No further details are available to provide an unbiased and factual description while maintaining conciseness.

Automatically generated - may contain errors

Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Lipofectamine 2000, by GenePharma (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Mir 431 5p mimic, by GenePharma (1 mentions) Mir 431 5p inhibitor, by GenePharma (1 mentions) Mir 205 5p mimic, by GenePharma (1 mentions) Mir 205 5p inhibitor, by GenePharma (1 mentions)

2 protocols using mle 12

Sepsis model using MLE-12 cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The alveolar epithelial cell line MLE-12 was purchased from the BeNa Culture Collection; Beijing Beina Chunglian Institute of Biotechnology. Cells were cultured in DMEM/F12 Coon's medium (DMEM/F12; Thermo Fisher Scientific, Inc.) containing 10% FBS (Thermo Fisher Scientific, Inc.) and maintained in a 5% CO₂ incubator at 37˚C. After the cells were passaged three times, cells in logarithmic phase were selected for follow-up assays.
The sepsis cell model was established using MLE-12 cells. Cells in the logarithmic phase were routinely cultured for 24 h at 37˚C. When the degree of cell fusion reached 90%, 100 ng/ml LPS (Sigma-Aldrich; Merck KGaA) was added to the culture for 24 h at 37˚C based on data from a previous study (18 (link)).

Wu K, & Wang L. (2021). Stomatin-knockdown effectively attenuates sepsis-induced oxidative stress and inflammation of alveolar epithelial cells by regulating CD36. Experimental and Therapeutic Medicine, 23(1), 69.

+ Open protocol

+ Expand

Influenza A Virus Infection in Lung Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mouse lung epithelial (MLE‐12) cell lines were purchased from the BeNa Culture Collection. Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 10% (v/v) fetal bovine serum (FBS), in a humidified atmosphere of 95% air‐ 5% CO₂ at 37°C. MLE‐12 cells were cultured in 6 well culture plate, and then the cells were transfected with miR‐205‐5p mimic, miR‐205‐5p inhibitor, miR‐431‐5p mimic, miR‐431‐5p inhibitor, NC or inhibitor negative control (INC), with lipofectamine 2000 for 48 h. miR‐205‐5p inhibitor, miR‐431‐5p inhibitor, INC were synthesized by Genepharma. These miRNA mimic (or respective inhibitor) transfected MLE‐12 cells were simultaneously infected with influenza A virus (MLE‐12 Infection model). The cells cultured in complete medium were considered as normal control. The culture plate was cultured in a 37°C, 5% CO₂ incubator. The cells were harvested 48 h after transfection, and Western blot was carried out using the primary antibodies against NP (Abcam) and GAPDH (Cell Signalling) as an internal control.²³

Bao Y., Shi Y., Zhou L., Gao S., Yao R., Guo S., Geng Z., Bao L., Zhao R, & Cui X. (2022). MicroRNA‐205‐5p: A potential therapeutic target for influenza A. Journal of Cellular and Molecular Medicine, 26(23), 5917-5928.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!